Related Experiment Video
Updated: Jan 2, 2026

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
Published on: January 27, 2012
Cellulose phosphorylation comparison and analysis of phosphorate position on cellulose fibers
Fleur Rol1, Cécile Sillard1, Michel Bardet2
1Univ. Grenoble Alpes, CNRS, Grenoble INP, LGP2, F-38000, Grenoble, France.
Abstract:
Chemical modifications of cellulose fibers as pretreatment for cellulose nanofibrils (CNF) production have been investigated to improve the production process and the quality of obtained cellulosic nanomaterial. In this study, phosphorylation of cellulose fibers was done in anticipation of a future nanofibrillation. Different phosphate salts, namely NH4H2PO4, (NH4)2HPO4, Na2HPO4, NaH2PO4 and LiH2PO4 with different constants of solubility (Ks) were used to increase the efficiency of the modification. Phosphorylated cellulose pulps were analyzed using elemental analysis, solid-state 13C and 31P NMR, or conductimetric titration method. No effect of Ks was observed whereas a counterion effect was pointed out. The study also reported the effect of pH, cellulose consistency, temperature and urea content in phosphorylation efficiency. Finally, chemical functionalization and penetration of phosphorylation reagents in the cellulose fibers were evaluated using XPS, SEM-EDX, ToF-SIMS and solid-state NMR.
More Related Videos
Related Concept Videos
Phosphorylation
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
ATP Energy Storage and Release
One example of energy coupling using ATP involves a...
Role of Microtubules in Cell Wall Deposition
Protein Kinases and Phosphatases
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
ATP Driven Pumps I: An Overview
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
Glycolysis: Preparatory Phase

