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Updated: Jan 2, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
Structure and bioactivity of sulfated α-D-mannan from marine bacterium Halomonas halocynthiae KMM 1376T
Maxim S Kokoulin1, Alina P Filshtein1, Lyudmila A Romanenko1
1G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 let Vladivostoku, Vladivostok, 690022, Russia.
Abstract:
Halomonas halocynthiae KMM 1376T is a Gram-negative bacterium that has been isolated from gill tissue of the ascidian Halocynthia aurantium. Mild acid hydrolysis of the lipopolysaccharide of H. halocynthiae KMM 1376T afforded an O-polysaccharide, which was studied by sugar analysis and NMR spectroscopy. The following structure of the O-polysaccharide presented as sulfated α-D-mannan was established: →2)-α-D-Manp3,6S-(1→3)-α-D-Manp2Ac(∼71%)6S-(1→3)-α-D-Manp-(1→. Study of biological activity has shown that sulfated α-D-mannan can specifically reduce the cell viability and colony formation of the human breast adenocarcinoma MDA-MB-231 cells in a concentration-dependent manner. In addition, polysaccharide inhibits epidermal growth factor induced neoplastic cell transformation in mouse epidermal JB6 Cl41 cells.
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