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Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture
Giovanni Giuseppe Giobbe1, Claire Crowley1, Camilla Luni2
1Stem Cell and Regenerative Medicine Section, University College London GOS Institute of Child Health, London, UK.
Nature Communications
|December 13, 2019
Summary
Extracellular matrix hydrogels from decellularized tissues support human organoid growth. This breakthrough advances regenerative medicine by enabling clinical applications for organoids derived from gastric, hepatic, pancreatic, and intestinal tissues.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Organoids show therapeutic promise but lack scalable, GMP-compliant expansion systems.
- Clinical translation of organoids is hindered by current culture limitations.
Purpose of the Study:
- To investigate extracellular matrix (ECM) hydrogels from decellularized tissues (DT) as a scaffold for organoid expansion.
- To assess the potential of these ECM hydrogels for clinical translation in regenerative medicine.
Main Methods:
- Decellularized porcine small intestine (SI) mucosa/submucosa was used to create ECM hydrogels.
- Human organoids (gastric, hepatic, pancreatic, SI) were derived and cultured within these gels.
- Transcriptomic stability and in vivo delivery potential were evaluated.
Main Results:
- ECM hydrogels supported the formation and growth of endoderm-derived human organoids.
- Organoid cultures maintained a stable transcriptomic signature within the ECM environment.
- ECM gels demonstrated potential for direct organoid derivation and in vivo delivery.
Conclusions:
- ECM hydrogels derived from decellularized tissues offer a promising solution for GMP-compliant organoid expansion.
- This approach facilitates the clinical application of human organoids in regenerative medicine.
- ECM-derived hydrogels enable stable organoid growth and versatile clinical use.
