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miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

11.2K

Circular RNA ITCH suppressed prostate cancer progression by increasing HOXB13 expression via spongy miR-17-5p

Xuegang Wang1, Rong Wang2, Zhun Wu1

  • 11Department of Urology, The First Affiliated Hospital of Xiamen University, The First Clinical College of Fujian Medical University, Xiamen, 361003 Fujian People's Republic of China.

Cancer Cell International
|December 13, 2019
PubMed
Abstract

Insights

Circular RNA Itchy E3 ubiquitin protein ligase (Circ-ITCH) is downregulated in prostate cancer (PC). Circ-ITCH targets miR-17-5p to regulate HOXB13, inhibiting PC progression and offering a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNA Itchy E3 ubiquitin protein ligase (Circ-ITCH) is downregulated in various cancers, but its role in prostate cancer (PC) is unclear.
  • Investigating Circ-ITCH's function in PC is crucial for understanding its potential as a therapeutic target.

Purpose of the Study:

  • To elucidate the functional role of Circ-ITCH in prostate cancer.
  • To investigate the regulatory axis of Circ-ITCH targeting miR-17-5p and HOXB13 in PC.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) and Western blotting were used to measure gene and protein expression.
  • Cell proliferation and apoptosis were assessed using CCK-8, colony formation, BrdU labeling, and flow cytometry.
  • Luciferase reporter assays identified downstream targets of miR-17-5p and Circ-ITCH.

Main Results:

  • Circ-ITCH expression was significantly reduced in PC tissues and cell lines, correlating with advanced tumor stage and Gleason score.
  • Circ-ITCH overexpression inhibited PC cell proliferation and promoted apoptosis.
  • Circ-ITCH acted as a molecular sponge for miR-17-5p, increasing HOXB13 expression and inhibiting PC progression.

Conclusions:

  • Circ-ITCH promotes prostate cancer progression via the HOXB13/miR-17-5p axis.
  • Circ-ITCH represents a potential therapeutic target for prostate cancer treatment.
Keywords:
CircITCHHOXB13ProliferationProstate cancermiR-17-5p

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