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Related Experiment Video

Updated: Jan 2, 2026

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device
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High-throughput three-dimensional spheroid tumor model using a novel stamp-like tool.

Wei Liao1, Jieqing Wang2, Jiecheng Xu1

  • 1Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou, China.

Journal of Tissue Engineering
|December 13, 2019
PubMed
Summary

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Researchers developed a low-cost, 3D-printed mold for spheroid culture, improving hepatocellular carcinoma cell models. This novel tool enhances 3D cell culture for research and drug screening.

Area of Science:

  • Biotechnology
  • Cancer Research
  • 3D Cell Culture

Background:

  • Spheroid culture mimics in vivo tumor structure, serving as a valuable research model.
  • Existing commercial spheroid culture tools are expensive and may yield suboptimal results for certain cell types.

Purpose of the Study:

  • To design and validate a cost-effective, reusable 3D-printed mold for spheroid culture.
  • To evaluate the efficacy of the homemade tool for hepatocellular carcinoma (HCC) spheroid formation and cell function.
  • To compare spheroid and 2D culture models for drug sensitivity testing in HCC.

Main Methods:

  • A 3D-printed, stamp-like resin mold was designed for creating microstructures on an agarose substrate in a 96-well plate.
  • The mold was used for spheroid formation of four HCC cell lines.
Keywords:
3D cultureagarosedrug screeningprimary hepatocarcinoma cellsspheroids

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  • Drug sensitivity assays were performed using patient-derived HCC cells in both spheroid and 2D cultures.

    • Main Results:

    • The homemade spheroid culture tool demonstrated superior spheroid formation compared to commercial ultralow attachment plates for HCC cells.
    • Three-dimensional culture enhanced HCC cell functions compared to traditional 2D culture.
    • Drug sensitivity testing revealed distinct patterns between spheroid and 2D cultures using patient-derived HCC cells.

    Conclusions:

    • The developed 3D-printed spheroid culture tool is affordable, reusable, and efficient for spheroid formation.
    • This technique improves cell function and offers a more relevant model for drug sensitivity testing in HCC.
    • The tool shows potential for personalized cancer treatment and high-throughput drug screening.