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Asparagine-linked oligosaccharides containing poly-N-acetyllactosamine chains are preferentially bound by immobilized
1Department of Biochemistry, School of Chemical Sciences, University of Georgia, Athens 30602.
The Journal of Biological Chemistry
|November 5, 1988
Abstract:
We have investigated the carbohydrate-binding specificity of a mammalian lectin, calf heart agglutinin, by determining the interaction of the immobilized lectin with a variety of complex-type Asn-linked oligosaccharides. Our results demonstrate that calf-heart agglutinin binds with high affinity to oligosaccharides containing the repeating disaccharide (3Gal beta 1-4GlcNAc beta 1)n or poly-N-acetyllactosamine sequence and that the presence of terminal beta-linked galactosyl residues is neither sufficient nor necessary for high affinity interactions.