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Related Experiment Videos

Platelet-derived growth factor regulates glucose transporter expression.

B J Rollins1, E D Morrison, P Usher

  • 1Division of Medicine, Dana-Farber Cancer Institute, Boston, Massachusetts.

The Journal of Biological Chemistry
|November 15, 1988
PubMed
Summary

Platelet-derived growth factor (PDGF) increases glucose transport by regulating the glucose transporter gene. This growth factor boosts glucose uptake and transporter levels, impacting cellular proliferation.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Regulated glucose transport is crucial for cellular proliferation.
  • Growth factors play a significant role in cellular processes.

Purpose of the Study:

  • To investigate the effect of platelet-derived growth factor (PDGF) on glucose transporter gene expression and function.
  • To determine if PDGF regulates glucose transport in mouse fibroblasts.

Main Methods:

  • Treatment of mouse fibroblasts with PDGF.
  • Measurement of glucose transport rates.
  • Quantification of membrane-associated glucose transporter protein.
  • Analysis of glucose transporter mRNA levels and gene transcription.
  • Experiments conducted in the presence of cycloheximide.

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Main Results:

  • PDGF increased glucose transport rate by 1.7-fold.
  • PDGF elevated membrane-associated glucose transporter protein by 1.7-fold.
  • PDGF caused a 5-fold increase in glucose transporter mRNA accumulation.
  • PDGF enhanced both gene transcription and mRNA stability.
  • Induction occurred independently of new protein synthesis (cycloheximide-insensitive).

Conclusions:

  • PDGF is a potent regulator of glucose transporter gene expression.
  • The glucose transporter is identified as a PDGF-inducible competence gene.
  • These findings highlight a mechanism linking growth factor signaling to metabolic regulation for cell growth.