Frankia soli sp. nov., an actinobacterium isolated from soil beneath Ceanothus jepsonii

Actinobacterial strain Cj^T was directly isolated from soil beneath Ceanothus jepsonii growing in the USA. The strain formed cell structures typical of the genus Frankia including extensive hyphae, vesicles and sporangia, and it effectively nodulated members of the actinorhizal Colletieae, Elaeagnaceae and Myricaceae. The whole-cell hydrolysate of strain Cj^T was rich in meso-diaminopimelic acid and galactose, glucose, mannose, xylose, ribose and a trace of rhamnose. Tbe polar lipid profile contained phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol and glycophospholipid. The menaquinone was predominantly MK-9(H₄). The fatty acid profile predominantly consisted of C_17 : 1ω8c, iso-C_16 : 0, C_15:0, C_16 : 0 and C_17 : 0. A multilocus sequence analysis phylogeny based on atp1, ftsZ, dnaK, gyrA and secA gene sequences positioned the strain within Elaeagnaceae- and Colletieae-nodulating species together with Frankia elaeagni DSM 46783^T, Frankia discariae DSM 46785^T and Frankia irregularis DSM 45899^T. Pairwise 16S rRNA gene sequence similarities showed that strain Cj^T was most closely related to F. discariae DSM 46785^T (99.78 %) while their digital DNA-DNA hybridization value was 41.1 %. Based on the overall analyses, strain Cj^T (=DSM 100623^T=CECT 9041^T) warrants classification as the type strain of a novel species, for which the name Frankia soli sp. nov. is proposed.