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Updated: Jan 2, 2026

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
Published on: March 16, 2017
The involvement of eukaryotic initiation factor 5A in trophoblast cell function
Xiaoli Qin1,2,3, Yan Chen1,2,3, Jiangjing Yuan1,2,3
1International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Eukaryotic translation initiation factor 5A (eIF5A) is crucial for trophoblast cell function. This study shows eIF5A regulates methyltransferase-like protein 14 (METTL14), impacting spontaneous abortion risk.
Area of Science:
- Reproductive biology
- Molecular genetics
- Cell biology
Background:
- Abnormal trophoblast cell growth and migration contribute to spontaneous abortion.
- The precise mechanism of eukaryotic translation initiation factor 5A (eIF5A) in trophoblast cells is not fully understood.
Purpose of the Study:
- To investigate the role of eIF5A in human chorionic trophoblast HTR8 cells.
- To identify genes regulated by eIF5A.
- To explore the potential of eIF5A and METTL14 as biomarkers for spontaneous abortion.
Main Methods:
- RNA sequencing (RNA-seq) to analyze transcriptome changes in eIF5A-knockdown HTR8 cells.
- Knockdown and overexpression experiments to assess the function of eIF5A and methyltransferase-like protein 14 (METTL14).
- Analysis of eIF5A and METTL14 expression in human abortion samples.
Main Results:
- eIF5A knockdown impaired HTR8 cell viability, proliferation, and migration.
- eIF5A regulates both mRNA and protein levels of METTL14.
- METTL14 inhibition mimicked the effects of eIF5A knockdown, and METTL14 overexpression rescued these effects.
- Reduced expression of eIF5A and METTL14 was observed in spontaneous abortion tissues.
Conclusions:
- eIF5A is essential for trophoblast cell function through its regulation of METTL14.
- The eIF5A-METTL14 pathway represents a potential therapeutic target and diagnostic marker for spontaneous abortion.
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