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A Method of Targeted Cell Isolation via Glass Surface Functionalization
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Fluorescence enhanced BA-LIFT for single cell detection and isolation.

A Marquez1, M Gómez-Fontela1,2, S Lauzurica1

  • 1Centro Láser, Universidad Politécnica de Madrid, Madrid, España, Spain.

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Summary
This summary is machine-generated.

A novel blister-actuated laser-induced forward transfer (BA-LIFT) technique enables precise bioprinting of living cells with high viability. This method utilizes a polyimide tape and imaging system for accurate cell selection and transfer.

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Area of Science:

  • Biotechnology
  • Laser-based cell manipulation
  • Tissue engineering

Background:

  • Laser bioprinting offers high precision for cell transfer.
  • Standard laser-induced forward transfer (LIFT) requires indirect methods to protect cells from direct laser irradiation.
  • An energy absorption layer is crucial for indirect LIFT techniques.

Purpose of the Study:

  • To introduce and characterize a novel blister-actuated laser-induced forward transfer (BA-LIFT) technique for cell bioprinting.
  • To integrate a vision system for enhanced cell selection and viability monitoring.
  • To establish the printability map and assess cell viability in hydrogels using BA-LIFT.

Main Methods:

  • Development of the blister-actuated laser-induced forward transfer (BA-LIFT) technique using a polyimide tape as an energy absorption layer.
  • Implementation of a coaxial in-line fluorescence and conventional imaging vision system for cell tracking and identification.
  • Studying the printability of sodium alginate and methylcellulose hydrogels across a range of laser fluences (6.1 to 2.0 J cm⁻²).

Main Results:

  • The BA-LIFT technique successfully bioprinted cells with high precision and viability (98% at low fluences).
  • Hydrogel concentration influences optimal laser fluence: less viscous hydrogels perform better at lower fluences, while more viscous ones require higher fluences.
  • The integrated vision system enabled tracking of primary cells and cell lines, confirming their integrity, proliferation, and functional activity post-transfer.

Conclusions:

  • The BA-LIFT technique, coupled with an imaging system, provides a precise and viable method for laser-based cell bioprinting.
  • The technique demonstrates successful isolation and tracking of specific cell types, including mouse hematopoietic progenitor stem cells and natural killer cells.
  • BA-LIFT offers a promising platform for applications in cell-based assays, regenerative medicine, and advanced tissue engineering.