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Related Experiment Videos

Analytical and physiological variability of salivary microbial counts.

C Bentley1, J J Crawford, C A Broderius

  • 1Department of Oral Diagnosis, School of Dentistry, University of North Carolina, Chapel Hill 27599.

Journal of Dental Research
|November 1, 1988
PubMed
Summary

Quantifying mutans streptococci (MS) and Lactobacillus in saliva is key for identifying dental caries risk. This study found sample collection methods significantly impact results, more than processing or short-term storage.

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Area of Science:

  • Microbiology
  • Dental Research
  • Clinical Diagnostics

Background:

  • Quantifying mutans streptococci (MS) and Lactobacillus aids in identifying patients at high risk for dental caries.
  • Understanding the variability of this diagnostic procedure is crucial for reliable risk assessment.

Purpose of the Study:

  • To investigate the analytical and physiological variability of quantifying MS and Lactobacillus for dental caries risk identification.
  • To determine factors influencing the precision of salivary microbial analysis.

Main Methods:

  • Repeated sampling studies to assess variability in specimen collection and processing.
  • Sample stability tests over 72 hours at various temperatures (5°C, room temperature, 37°C).
  • Serial analyses on individuals to evaluate physiological variability, including diurnal and day-to-day variations.

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Main Results:

  • Sample collection was the primary source of imprecision in salivary microbial analysis.
  • Sample processing showed minimal variability (CVs of 2.3% for MS, 2.1% for Lactobacillus).
  • Samples were stable for up to 72 hours, with minor lactobacilli viability loss at higher temperatures/longer storage.
  • Morning samples (rising) showed higher counts than post-breakfast/brushing samples.
  • Considerable day-to-day variability was observed, with 95% confidence limits exceeding 1 log in 28% (MS) and 39% (Lactobacillus) of data sets.

Conclusions:

  • Salivary microbial analysis for caries risk is highly sensitive to sample collection techniques.
  • While sample processing and short-term storage are relatively stable, physiological and day-to-day variations are significant.
  • Standardized saliva collection protocols are essential to improve the reliability of dental caries risk assessment using MS and Lactobacillus counts.