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Related Experiment Video

Updated: Jan 2, 2026

In Vivo Imaging Uncovers the Migratory Behavior of Leukocytes within the Joints

In Vivo Imaging Uncovers the Migratory Behavior of Leukocytes within the Joints

Published on: December 9, 2025

Exploring inflammatory signatures in arthritic joint biopsies with Spatial Transcriptomics.

Konstantin Carlberg^1,2, Marina Korotkova², Ludvig Larsson¹

¹Department of Gene Technology, Royal Institute of Technology, Science for Life Laboratory, Stockholm, Sweden.

Scientific Reports

|December 14, 2019

Summary

This summary is machine-generated.

Spatial transcriptomics reveals distinct immune profiles in rheumatoid arthritis (RA) and spondyloarthritis (SpA) synovial tissues. RA shows adaptive immune responses and T-B cell interactions, while SpA implicates tissue repair mechanisms.

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Area of Science:

Immunology
Molecular Biology
Rheumatology

Background:

Analyzing transcriptomic profiles in tissue sections while retaining cellular context is now feasible.
Rheumatoid arthritis (RA) and spondyloarthritis (SpA) are chronic inflammatory diseases affecting joints.

Purpose of the Study:

To explore synovial biopsies from RA and SpA patients using Spatial Transcriptomics (ST).
To provide an unbiased mRNA study at the inflammation site in these chronic inflammatory diseases.

Main Methods:

Spatial Transcriptomics (ST) was used on synovial tissue biopsies.
Differential gene expression analysis (DEA), pathway analysis, and Xcell immune cell type identification were performed.
Immunohistochemistry (IHC) was used for validation.

Main Results:

RA biopsies showed an adaptive immune response profile with T-B cell interactions.
SpA biopsies implicated functions associated with tissue repair.
RA displayed central memory T cells, while SpA showed prominent effector memory T cells.

Conclusions:

Spatial transcriptomics enables a deeper understanding of cellular mechanisms and diversity in chronic inflammatory diseases.
Distinct molecular profiles were identified in RA and SpA synovial tissues.