Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

6.4K
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
6.4K
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

3.2K
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A proliferation-inducing ligand enhances the anti-tumor effect of BCMA CAR T-cell through inhibition of soluble BCMA.

Biochimica et biophysica acta. Molecular basis of disease·2026
Same author

Regulatory B-cell states in NSCLC immunotherapy resistance: mechanisms, spatial context and translational implications.

Cancer immunology, immunotherapy : CII·2026
Same author

Dissociable peripheral and central mechanisms of monoacylglycerol lipase inhibition on pain- and depression-related behaviors in a rat model of neuropathic pain.

Pain·2026
Same author

Arthroscopic Treatment of Patellar Clunk Syndrome After Total Knee Arthroplasty.

Arthroplasty today·2026
Same author

A novel fully visualized transparent channel in posterior uniportal endoscopic spine surgery: a paradigm shift for nerve root exposure and decompression under direct visualization.

Journal of neurosurgery. Spine·2026
Same author

Decoding α-MoC<sub>1-</sub> <sub>x</sub> Nanoparticle Formation in Continuous Flow via Machine Learning.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Parallelized contactless microfluidic dispenser with superhydrophobic nozzles for scalable combinatorial screening.

Biomicrofluidics·2026
Same journal

Time resolved luminescence of millisecond lifetime dyes in droplet microfluidic systems.

Biomicrofluidics·2026
Same journal

Emerging trends in functional molecularly imprinted polymers for electrochemical detection of biomarkers.

Biomicrofluidics·2025
Same journal

Deep learning assisted mechanotyping of individual cells through repeated deformations and relaxations in undulating channels.

Biomicrofluidics·2025
Same journal

<i>Giardia</i> purification from fecal samples using rigid spiral inertial microfluidics.

Biomicrofluidics·2025
Same journal

Point of care sepsis diagnosis: Exploring microfluidic techniques for sample preparation, biomarker isolation, and detection.

Biomicrofluidics·2025
See all related articles

Related Experiment Video

Updated: Jan 2, 2026

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals
09:58

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Published on: May 10, 2018

10.0K

Spectrophotometry in modular microfluidic architectures.

Bryant Thompson, Krisna C Bhargava1, Alexander T Czaja

  • 1ReoLab Inc., Pasadena, California 91103, USA.

Biomicrofluidics
|December 14, 2019
PubMed
Summary
This summary is machine-generated.

This study presents a 3D-printed microfluidic system for assaying bladder cancer biomarkers using gold nanoparticle aggregation and spectrophotometry. The innovative approach offers comparable performance to traditional methods at a competitive cost.

More Related Videos

Author Spotlight: Characterization of Low-Affinity Protein Interactions in Solution Using MassFluidix Technology
06:39

Author Spotlight: Characterization of Low-Affinity Protein Interactions in Solution Using MassFluidix Technology

Published on: January 26, 2024

2.8K
Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

11.2K

Related Experiment Videos

Last Updated: Jan 2, 2026

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals
09:58

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Published on: May 10, 2018

10.0K
Author Spotlight: Characterization of Low-Affinity Protein Interactions in Solution Using MassFluidix Technology
06:39

Author Spotlight: Characterization of Low-Affinity Protein Interactions in Solution Using MassFluidix Technology

Published on: January 26, 2024

2.8K
Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

11.2K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Chemical biomarker assays are crucial for noninvasive disease assessment, often using colorimetric reactions or spectrophotometry.
  • Integrating spectrophotometry into microfluidic devices for these assays remains a significant technical challenge.
  • Spectrophotometry offers advantages in biomarker assay development due to ease of acquisition, mechanism design, and sensitivity tuning.

Purpose of the Study:

  • To develop a novel microfluidic system for assaying hyaluronidase, a urinary biomarker for bladder cancer.
  • To integrate 3D-printed discrete microfluidic elements with absorbance spectrometry for biomarker quantification.
  • To demonstrate the system's performance and cost-effectiveness compared to conventional methods.

Main Methods:

  • Utilized 3D-printed discrete microfluidic elements to construct a model system.
  • Employed gold nanoparticle aggregation and absorbance spectrometry for hyaluronidase assaying.
  • Compared the performance of the microfluidic system against laboratory microtiter plate-based techniques.

Main Results:

  • The developed microfluidic system successfully assayed hyaluronidase, a biomarker for bladder cancer.
  • Absorbance spectrometry of gold nanoparticle aggregation was effectively implemented within the microfluidic workflow.
  • The system demonstrated equivalent performance to traditional microtiter plate assays.

Conclusions:

  • 3D-printed microfluidic elements can be leveraged to create effective spectrophotometric assay systems.
  • This approach offers a competitive and cost-effective alternative for biomarker quantification in disease assessment.
  • The model system shows promise for advancing noninvasive diagnostic tools for bladder cancer.