Relationship between retinal vessel tortuosity and oxygenation in sickle cell retinopathy

Reduced retinal vascular oxygen (O₂) content causes tissue hypoxia and may lead to development of vision-threatening pathologies. Since increased vessel tortuosity is an early sign for some hypoxia-implicated retinopathies, we investigated a relationship between retinal vascular O₂ content and vessel tortuosity indices.

Dual wavelength retinal oximetry using a commercially available scanning laser ophthalmoscope was performed in both eyes of 12 healthy (NC) and 12 sickle cell retinopathy (SCR) subjects. Images were analyzed to quantify retinal arterial and venous O₂ content and determine vessel tortuosity index (VTI) and vessel inflection index (VII) in circumpapillary regions. Linear mixed model analysis was used to determine the effect of disease on vascular O₂ content, VTI and VII, and relate vascular O₂ content with VTI and VII. Models accounted for vessel type, fellow eyes, age and mean arterial pressure.

Retinal arterial and venous O₂ content were lower in SCR (O_2A = 11 ± 4 mLO₂/dL, O_2V = 7 ± 2 mLO₂/dL) compared to NC (O_2A = 18 ± 3 mLO₂/dL, O_2V = 13 ± 3 mLO₂/dL) subjects (p < 0.001). As expected, O₂ content was higher in arteries (15 ± 5 mLO₂/dL) than veins (10 ± 4 mLO₂/dL) (p < 0.001), but not different between eyes (OD: 12 ± 5 mLO₂/dL; OS:13 ± 5 mLO₂/dL) (p = 0.3). VTI was not significantly different between SCR (0.18 ± 0.07) and NC (0.15 ± 0.04) subjects, or between arteries (0.18 ± 0.07) and veins (0.16 ± 0.04), or between eyes (OD: 0.18 ± 0.07, OS:0.17 ± 0.05) (p ≥ 0.06). VII was significantly higher in SCR (10 ± 2) compared to NC subjects (8 ± 1) (p = 0.003). VII was also higher in veins (9 ± 2) compared to arteries (8 ± 5) (p = 0.04), but not different between eyes (OD: 9 ± 2; OS: 9 ± 2) (p = 0.2). There was an inverse linear relationship between vascular O₂ (13 ± 5 mLO₂/dL) content and VII (9 ± 2) (β = -0.5; p = 0.02).

The findings augment knowledge of relationship between retinal vascular oxygenation and morphological changes and potentially contribute to identifying biomarkers for assessment of retinal hypoxia due to SCR and other retinopathies.