Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

936
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
936
Magnetic Resonance Imaging01:24

Magnetic Resonance Imaging

8.9K
Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...
8.9K
Structural Isomerism02:34

Structural Isomerism

21.4K
Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...
21.4K
Labeling DNA Probes03:31

Labeling DNA Probes

9.1K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.1K
Stereoisomerism02:52

Stereoisomerism

13.7K
Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula.
Transition metal complexes often exist as geometric isomers, in which the same atoms are connected through the same types of bonds but with differences in their orientation in space. Coordination complexes with two different ligands in the cis and trans positions from a ligand of interest form isomers. For example, the octahedral [Co(NH3)4Cl2]+ ion has two isomers (Figure 1) In the cis...
13.7K
Imaging Studies IV: Magnetic Resonance Imaging01:27

Imaging Studies IV: Magnetic Resonance Imaging

199
Introduction:Magnetic Resonance Imaging, or MRI, can include a specialized imaging technique of the urinary system known as Magnetic Resonance Urography (MRU). This radiation-free technique uses strong magnetic fields and radio waves to produce detailed images with the help of a computer. MRU is particularly effective for visualizing fluid-filled structures like the kidneys, ureters, and bladder.Applications of MRI in the Genitourinary SystemKidneys and Ureters: MRI detects tumors, cysts,...
199

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inner- and Second-Sphere Interactions of Co(II) and Fe(II) Parashift Agents with Lactate, Trifluorolactate, and Fluoride.

Inorganic chemistry·2026
Same author

Cytotoxicity and immunogenic cell death studies of water-soluble tetrahedral Ga(iii) or Fe(iii) coordination cages containing a Au(i) anticancer drug as guest.

Inorganic chemistry frontiers·2026
Same author

Octahedral Coordination Cages of Co(II) and Ni(II): Enhanced Probe Signal Through Symmetry.

Inorganic chemistry·2026
Same author

Deuterium magnetic resonance imaging of tumors using low-dose systemic deuterated water labeling.

Frontiers in oncology·2026
Same author

Genetic Analysis of Choroideremia-Related Rab Escort Proteins.

International journal of molecular sciences·2025
Same author

Zn(II)-Responsive MRI Probe Based on an Fe(III) Macrocyclic Complex.

Inorganic chemistry·2024

Related Experiment Video

Updated: Jan 2, 2026

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging
13:21

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

Published on: July 21, 2011

15.3K

Isomeric Co(ii) paraCEST agents as pH responsive MRI probes.

Christopher J Bond1, Roy Cineus, Alexander Y Nazarenko

  • 1Department of Chemistry, Natural Sciences Complex, University at Buffalo, the State University of New York, Amherst, NY 14260, USA. jmorrow@buffalo.edu.

Dalton Transactions (Cambridge, England : 2003)
|December 14, 2019
PubMed
Summary

A new cobalt(II) complex isomer, CCRM, generates four paramagnetically shifted CEST peaks. This isomer acts as an excellent pH probe in solutions and tissues due to its amide protons.

More Related Videos

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance
14:01

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance

Published on: July 22, 2011

15.0K
Synthesis of 68Ga Core-doped Iron Oxide Nanoparticles for Dual Positron Emission Tomography /T1Magnetic Resonance Imaging
07:26

Synthesis of 68Ga Core-doped Iron Oxide Nanoparticles for Dual Positron Emission Tomography /T1Magnetic Resonance Imaging

Published on: November 20, 2018

6.9K

Related Experiment Videos

Last Updated: Jan 2, 2026

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging
13:21

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

Published on: July 21, 2011

15.3K
Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance
14:01

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance

Published on: July 22, 2011

15.0K
Synthesis of 68Ga Core-doped Iron Oxide Nanoparticles for Dual Positron Emission Tomography /T1Magnetic Resonance Imaging
07:26

Synthesis of 68Ga Core-doped Iron Oxide Nanoparticles for Dual Positron Emission Tomography /T1Magnetic Resonance Imaging

Published on: November 20, 2018

6.9K

Area of Science:

  • Coordination Chemistry
  • Biophysical Chemistry
  • Chemical Biology

Background:

  • Paramagnetically shifted chemical exchange saturation transfer (CEST) agents offer sensitive detection methods.
  • Cobalt(II) complexes are explored for their potential in imaging and sensing applications.
  • Kinetic inertness and stable metal centers are crucial for developing reliable probes.

Purpose of the Study:

  • To synthesize and characterize a novel isomer of a cobalt(II) macrocyclic complex.
  • To evaluate the potential of this new isomer as a pH probe using CEST.
  • To investigate the relationship between the complex's structure and its pH-sensing capabilities.

Main Methods:

  • Synthesis and characterization of the cobalt(II) isomer (CCRM).
  • Measurement of chemical exchange saturation transfer (CEST) spectra.
  • Analysis of paramagnetically shifted peaks and amide proton exchange rates.
  • pH-dependent studies in solution and tissue homogenates.

Main Results:

  • A new Co(ii) isomer, CCRM, was synthesized, exhibiting increased kinetic inertness.
  • Four highly paramagnetically shifted CEST peaks were observed.
  • The isomer demonstrated excellent performance as a pH probe in both solution and tissue homogenates.
  • A ratiometric pH readout and pH-correlated amide proton exchange rate constants were obtained.

Conclusions:

  • The novel Co(ii) isomer (CCRM) is a promising candidate for pH sensing applications.
  • The observed CEST signals and pH-dependent properties highlight its utility in biological systems.
  • The trans-arrangement of 1,8-pendants contributes to the complex's kinetic inertness and stability.
  • This work advances the development of paramagnetic CEST agents for biomedical research.