Related Experiment Video
Updated: Jan 2, 2026
Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana
Published on: March 17, 2023
Cytochalasins from endophytic Diaporthe sp. GDG-118
Xishan Huang1, Dexiong Zhou1, Yan Liang1
1State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, P. R. China.
Abstract:
The plant Sophora tonkinensis, possessed a range of active compounds, was traditionally used in the medicine of Chinese minorities. Endophytic fungi were isolated from this plant, of which the fungus Diaporthe sp. GDG-118 was fermented and extracted with methanol. The extract was screened by antifungal and antibacterial assays leading to the discovery of two new 21-acetoxycytochalasins (1-2) and five known cytochalasins (3-7). These two new compounds were elucidated by spectroscopic analyses, and further their absolute configurations were determined by the X-ray of compound 3 and comparing their experimental CD spectra. The antibacterial and antifungal effects of these compounds were evaluated. Compound 2 showed significant inhibitory activity against Bacillus anthraci and Escherichia coli with MIC value of 12.5 μg/mL, and 7 showed strong antifungal activity against Alternaria oleracea, Pestalotiopsis theae and Colletotrichum capsici with MIC values of 3.125, 1.56 and 1.56 μg/mL, respectively.
