Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The Ras Gene

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Case of Breast Papillary Carcinoma in an Elderly Black Male Patient With Pathology, Ultrasound, and Mammogram Imaging Findings.

Cureus·2024

Same author

IASLC grading system predicts distant metastases for resected lung adenocarcinoma.

Journal of clinical pathology·2024

Same author

Single-cell sequencing reveals the landscape of the human brain metastatic microenvironment.

Communications biology·2023

Same author

Cardiac Involvement by Human Herpesvirus 8-Positive Diffuse Large B-Cell Lymphoma: An Unusual Presentation in a Patient with Human Immunodeficiency Virus.

Case reports in pathology·2022

Same author

Multiple endocrine neoplasia 2A presenting in a family with a history of Hirschprung's disease.

Oxford medical case reports·2022

Same author

Bulk and Single-Cell Profiling of Breast Tumors Identifies TREM-1 as a Dominant Immune Suppressive Marker Associated With Poor Outcomes.

Frontiers in oncology·2021

Search research articles

Related Experiment Video

Updated: Jan 2, 2026

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Published on: June 7, 2019

Rapid BRAF Mutation Testing in Pigmented Melanomas.

Danielle R Petty¹, Omer A Hassan, Callie S Barker

¹Department of Pathology, Wake Forest Baptist Medical Center, Winston-Salem, NC.

The American Journal of Dermatopathology

|December 14, 2019

Summary

This summary is machine-generated.

The Idylla BRAF assay accurately detects BRAF mutations in melanoma, even with high melanin content. This rapid molecular test provides reliable results for guiding targeted therapy decisions in advanced melanoma.

More Related Videos

Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines

Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines

Published on: October 8, 2015

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

Published on: November 13, 2012

Related Experiment Videos

Last Updated: Jan 2, 2026

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Published on: June 7, 2019

Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines

Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines

Published on: October 8, 2015

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

Published on: November 13, 2012

Area of Science:

Oncology
Molecular Diagnostics
Genetics

Background:

BRAF mutations occur in 40%-60% of melanomas, making their detection crucial for targeted therapy.
Melanin in melanoma samples can inhibit standard molecular assays like polymerase chain reaction (PCR).
A rapid and accurate BRAF mutation assay is needed for timely clinical decision-making in metastatic melanoma.

Purpose of the Study:

To evaluate the performance of the cartridge-based Idylla platform for BRAF mutation detection in melanoma.
To assess the impact of melanin content on the accuracy and reliability of the Idylla BRAF assay.
To determine the sensitivity, specificity, and turnaround time of the Idylla BRAF assay.

Main Methods:

The Idylla BRAF assay was tested on 23 melanoma samples, including resections, biopsies, and cell blocks.
Pathologists scored samples for melanin content (0-2 scale).
Results were compared against reference laboratory testing.

Main Results:

The Idylla assay successfully tested samples with high melanin content (pigment score 2).
Sensitivity and specificity for BRAF mutation detection were both 100% compared to reference testing.
The assay required minimal tissue input (detecting mutations in ~400 tumor cells) and had a total assay time of under 2 hours.

Conclusions:

The Idylla BRAF mutation assay is a rapid, robust, and accurate method for melanoma testing, unaffected by high melanin content.
The assay demonstrates high sensitivity and specificity, making it suitable for diverse clinical settings, including those with limited sample material.
Its speed and ease of use facilitate prompt BRAF mutation status determination to guide patient care.