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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Exon Recombination

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...

Chromatin Structure Regulates pre-mRNA Processing

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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Related Experiment Video

Updated: Jan 2, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Alternative splicing in aging and longevity.

Malini Bhadra¹, Porsha Howell¹, Sneha Dutta¹

¹Department of Molecular Metabolism, Harvard T.H. Chan School of Public Health, Boston, MA, 02115, USA.

|December 14, 2019

Summary

This summary is machine-generated.

RNA splicing, a key regulator of gene expression, is increasingly recognized as a hallmark of aging. Dysfunctional splicing contributes to aging, while interventions targeting splicing factors may extend lifespan.

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Area of Science:

Molecular Biology
Genetics
Gerontology

Background:

Alternative pre-mRNA splicing enhances proteomic diversity from genomic sequences.
Splicing dysregulation is linked to numerous diseases.
Recent evidence connects splicing to aging and longevity interventions.

Purpose of the Study:

To review RNA splicing as a hallmark of aging.
To explore splicing's role in aging phenotypes and longevity.
To discuss splicing profiles as biomarkers of biological age.

Main Methods:

Literature review of recent research on RNA splicing and aging.
Analysis of dysfunctional alternative splicing events across species.
Investigation of nutrient signaling pathways' impact on splicing factors.

Main Results:

Dysfunctional alternative splicing contributes to aging phenotypes.
Specific splicing factors are required for pro-longevity interventions.
Nutrient signaling pathways regulate splicing factor activity.
Splicing profiles show potential as predictors of biological age.

Conclusions:

RNA splicing is a critical factor in the aging process.
Understanding splicing alterations in aging may lead to therapeutics for extending healthy lifespan.
Splicing modulation presents a novel target for anti-aging strategies.