Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Editing02:23

RNA Editing

9.7K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.7K
MicroRNAs01:22

MicroRNAs

3.7K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.7K
MicroRNAs01:22

MicroRNAs

23.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.8K
RNA Interference01:23

RNA Interference

27.6K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.6K
Experimental RNAi02:15

Experimental RNAi

7.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.2K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

18.2K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

S100A10-ANXA2 tetramer inhibition hampers hepatic stellate cell activation in human MASLD organoids.

EMBO molecular medicine·2026
Same author

Paracellular transport along the nephron in physiology and pathophysiology.

Current opinion in nephrology and hypertension·2026
Same author

Hepatoprotective role for ERMP1 in MASLD-driven hepatocarcinogenesis and β-catenin-mutated tumors.

Cancer letters·2026
Same author

Derepression of the epithelial transcription factor GRHL2 promotes direct hepatocyte-to-cholangiocyte transdifferentiation.

PLoS biology·2025
Same author

Alcohol-related hepatitis induces a specific fibrosis profile through YAP activation in myofibroblasts.

JHEP reports : innovation in hepatology·2025
Same author

Letter to the Editor to "G3BP1, a stress granule core protein, ameliorates metabolic dysfunction-associated fatty liver disease by attenuating hepatocyte lipid deposition".

Diabetes, obesity & metabolism·2025

Related Experiment Video

Updated: Jan 1, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

2.9K

Deciphering miRNAs' Action through miRNA Editing.

Marta Correia de Sousa1, Monika Gjorgjieva1, Dobrochna Dolicka1

  • 1Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, CH-1211 Geneva, Switzerland.

International Journal of Molecular Sciences
|December 15, 2019
PubMed
Summary

MicroRNA (miRNA) editing, a process altering nucleotide sequences, significantly impacts gene regulation and disease development. Understanding this mechanism is key to developing new miRNA-targeted therapies for conditions like cancer and metabolic diseases.

Keywords:
ADARsAPOBECsmiRNA editingmicroRNA regulation

More Related Videos

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.7K
A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

2.9K

Related Experiment Videos

Last Updated: Jan 1, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

2.9K
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.7K
A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

2.9K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are crucial regulators of gene expression involved in numerous physiological and pathological processes.
  • The precise roles of miRNAs are complex and challenging to decipher due to cell-specific regulation and dependence on organismal status.
  • Factors beyond miRNA expression levels, such as RNA editing, influence miRNA activity and bioavailability.

Purpose of the Study:

  • To explore the regulatory mechanism of RNA editing in microRNA (miRNA) biogenesis and activity.
  • To elucidate how nucleotide modifications within miRNAs affect their stability, maturation, and target specificity.
  • To understand the implications of miRNA editing in stress responses and diseases like cancer and metabolic disorders.

Main Methods:

  • Review of current literature on miRNA editing mechanisms.
  • Analysis of enzymes involved in adenosine/cytidine deamination of primary miRNAs.
  • Examination of how nucleotide modifications impact miRNA function and target interactions.

Main Results:

  • RNA editing involves enzymes that introduce nucleotide changes (e.g., A-to-I, C-to-U) in primary miRNAs.
  • These modifications critically affect miRNA stability, maturation, and specificity towards messenger RNAs (mRNAs).
  • Editing events can alter miRNA-mediated regulation of cellular stress responses and disease development.

Conclusions:

  • RNA editing is a vital regulatory mechanism controlling miRNA function beyond expression levels.
  • Understanding miRNA editing deepens insights into complex diseases and facilitates the development of novel miRNA-based therapeutics.
  • Further research into miRNA editing is essential for advancing molecular medicine and targeted therapies.