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Updated: Jan 1, 2026

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
Published on: August 21, 2021
Mobility and Repair of Damaged DNA: Random or Directed?
Roxanne Oshidari1, Karim Mekhail2, Andrew Seeber3
1Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, MaRS Centre West Tower, 661 University Avenue, Toronto, ON, M5G 1M1, Canada.
Abstract:
The increased mobility of damaged DNA within the nucleus can promote genome stability and cell survival. New cell biology approaches have indicated that damaged DNA mobility exhibits random and directed movements during DNA repair. Here, we review recent studies that collectively reveal that cooperation between different molecular mechanisms, which underlie increases in the random and directional motion of damaged DNA, can promote genome repair. We also review the latest approaches that can be used to distinguish between random and directed motions of damaged DNA or other biological molecules. Detailed understanding of the mechanisms behind the increased motion of damaged DNA within the nucleus will reveal more of the secrets of genome organization and stability while potentially pointing to novel research and therapeutic tools.
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