Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Brain slice glucose utilization.

G C Newman1, F E Hospod, C S Patlak

  • 1Department of Neurology, State University of New York, Stony Brook.

Journal of Neurochemistry
|December 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimizing dynamic T2* MR imaging for measurement of cerebral blood flow using infusions for cerebral blood volume.

AJNR. American journal of neuroradiology·2006
Same author

Cerebral blood volume measurements by T*2-weighted MRI and contrast infusion.

Magnetic resonance in medicine·2003
Same author

Fluid-attenuated inversion recovery and diffusion- and perfusion-weighted MRI abnormalities in 117 consecutive patients with stroke symptoms.

Stroke·2001
Same author

A dose of 75 microg/kg/d of i.v. anti-D increases the platelet count more rapidly and for a longer period of time than 50 microg/kg/d in adults with immune thrombocytopenic purpura.

British journal of haematology·2001
Same author

Effects of postnatal dexamethasone on blood-brain barrier permeability and brain water content in newborn lambs.

American journal of physiology. Regulatory, integrative and comparative physiology·2001
Same author

Effects of duration of positive-pressure ventilation on blood-brain barrier function in premature lambs.

Journal of applied physiology (Bethesda, Md. : 1985)·2000
Same journal

A PARK9 iPSC-Derived Dopaminergic Neuron Model Enables Drug Screening Targeting Autophagy-Lysosome Pathway Dysfunction in Parkinson's Disease.

Journal of neurochemistry·2026
Same journal

Opposing Estrous Cycle-Dependent Norepinephrine and Dopamine Regulation in Response to Methamphetamine.

Journal of neurochemistry·2026
Same journal

Exercise Snacking in Alzheimer's Disease: A Mechanistic Rationale Based on Repeated Exerkine Signaling.

Journal of neurochemistry·2026
Same journal

The Converging Effects of Different Categories of Antidepressants on the Brain: A Systematic Meta-Analysis of Public Transcriptional Profiling Data From the Hippocampus and Cortex.

Journal of neurochemistry·2026
Same journal

Splice Type-Specific Effects of Gαo Subunits on Cerebellar Anatomy and Synapse Formation.

Journal of neurochemistry·2026
Same journal

Neurobiological and Behavioral Consequences of Prenatal Methadone or Buprenorphine Exposure: Insight From Animal Models.

Journal of neurochemistry·2026
See all related articles

This study investigated 2-deoxyglucose metabolism in hypothalamic brain slices, revealing insights into glucose utilization and its diurnal variations. The findings support the use of 1,000-micron slices as a model for brain ischemia.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Metabolic Research

Background:

  • Understanding brain glucose metabolism is crucial for neurological research.
  • In vitro models are essential for studying complex brain functions and diseases.
  • Previous studies have utilized brain slices, but detailed kinetic analysis of 2-deoxyglucose metabolism is ongoing.

Purpose of the Study:

  • To analyze the metabolism of 2-deoxyglucose (2DG) in hypothalamic brain slices of varying thickness (540 and 1,000 microns).
  • To determine kinetic parameters, including the lumped constant (LC), for 2DG uptake and metabolism.
  • To compare in vitro findings with existing in vivo data and validate the use of brain slices as a model for brain ischemia.

Main Methods:

  • Studied 2-deoxyglucose (2DG) and 2-deoxyglucose-6-phosphate (2DG6P) levels in hypothalamic brain slices.

Related Experiment Videos

  • Employed nonlinear least-squares methods to analyze uptake and washout kinetics for kinetic modeling.
  • Measured total slice radioactivity (Ci*) and utilized image analysis of autoradiograms for glucose utilization assessment.

    • Main Results:

    • Identified a small, slowly decaying 2DG component not predicted by standard models.
    • Demonstrated a strong correlation between whole-slice homogenization and autoradiogram image analysis for glucose utilization.
    • Observed spontaneous diurnal variation in in vitro glucose utilization in the suprachiasmatic nucleus, matching in vivo data.
    • Found increased in vitro glucose utilization (approx. 60%) in 1,000-micron slices compared to 540-micron slices, with altered kinetic constants (LC, k1, k3).

    Conclusions:

    • The 1,000-micron hypothalamic slice is a viable in vitro model for studying brain ischemia without infarction.
    • Kinetic modeling of 2DG metabolism provides valuable insights into brain slice physiology.
    • In vitro glucose utilization in hypothalamic slices exhibits diurnal variations consistent with in vivo observations.