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Updated: Jan 1, 2026

Sensitive Detection of Proteopathic Seeding Activity with FRET Flow Cytometry
Published on: December 8, 2015
Leaky endosomes push tau over the seed limit
Chris Ugbode1, Laura Fort-Aznar1, Sean T Sweeney2
1Department of Biology, University of York, York YO10 5DD, United Kingdom.
Abstract:
The inter- and intracellular propagation of aggregated proteins like tau is emerging as a central mechanism behind progression of various neurodegenerative diseases. The steps by which tau aggregates and propagates is currently unclear. Chen et al. now combine a cell-based model of tau aggregation with a CRISPR interference (CRISPRi) genetic screen to identify components of the endosomal sorting complex required for transport (ESCRT) machinery as mediators of intracellular propagation of tau aggregates. These findings reveal a role for endolysosomal integrity in blocking tau propagation.
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