A graphene quantum dots-Pb²⁺ based fluorescent switch for selective and sensitive determination of D-penicillamine

Taking consideration of metal-induced fluorescence quenching and excellent coordination effect of D-penicillamine (D-PA), a graphene quantum dots (GQDs)-based fluorescent switch for D-PA detection was designed and established firstly with the help of lead ions. GQDs obtained from citric acids made them rich in carboxyl and hydroxyl groups, giving GQDs the ability to combine with lead ions. As anticipated, the fluorescence intensity was quenched by Pb²⁺ through electron transfer process. Further, the addition of D-PA effectively recovered the fluorescence due to the departure of Pb²⁺ from GQDs aroused by the strong coordination between D-PA and Pb²⁺. Thus, a fluorescent switch was activated for D-PA detection. The fluorescence recovery efficiencies were found to be proportional to the concentration of D-PA in the range of 0.6-50 μmol L^-1 and the detection limit was 0.47 μmol L^-1. The real sample detection was performed in human urea sample and satisfactory recoveries of 96.84%-102.13% were obtained. The GQDs-Pb²⁺ based fluorescent switch sensing method was firstly established with low detection limit and wide linear range, making it a supplement and improvement for D-PA detection.