Related Experiment Video

Updated: Jan 1, 2026

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
08:42

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Published on: July 3, 2020

4.9K

Critical Roles of E2F3 in Growth and Musculo-skeletal Phenotype in Mice

Hae-Rim Kim1, Faiz Ur Rahman1, Kwang-Soo Kim1,2

  • 1Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology, Yeonjudanji-ro 30, Chungbuk 28116, Korea.

International Journal of Medical Sciences
|December 17, 2019
PubMed

Insights

The E2F3 gene is crucial for normal mouse growth, impacting muscle and bone development. Ablating E2F3 in mice caused growth retardation and skeletal issues, highlighting its importance in development.

Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • E2F3 is a key regulator of the cell cycle and proliferation.
  • It targets retinoblastoma (RB) proteins, which are tumor suppressors.

Purpose of the Study:

  • To investigate the in vivo role and function of E2F3.
  • To examine phenotypic abnormalities resulting from E2F3 gene deletion.

Main Methods:

  • Deletion of the E2f3 gene in mice.
  • Phenotypic analysis of E2f3-ablated mice on a C57BL/6N background.

Main Results:

  • Complete ablation of E2F3 was fully penetrant in C57BL/6N mice.
  • E2f3-deficient mouse embryos developed without fatal disorders.
  • Mice lacking E2F3 showed reduced body weight, growth retardation, skeletal imperfections, and poor grip strength.

Conclusions:

  • E2F3 plays a pivotal role in muscle and bone development.
  • E2F3 is essential for normal mouse growth and development.
Keywords:
DevelopmentE2f3+/- KnockoutGrowth retardationMouse phenotypeskeletal imperfection

More Related Videos

Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification07:23

Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification

Published on: December 3, 2016

12.4K
Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells14:36

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

Published on: September 21, 2010

29.2K

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.6K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.6K