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Updated: Jan 1, 2026

Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
The Cell Cycle G2/M Block Is an Indicator of Cellular Radiosensitivity
Chang Liu1, Jing Nie2,3, Rensheng Wang1
1Department of Radiotherapy, the First Affiliated Hospital of Guangxi Medical University, Nanning, China.
Background:
Determination of the radiosensitivity of a specific tumor is essential to its precision tumor radiotherapy, but the measurement of cellular radiosensitivity with a routine colony forming assay is both labor- and time-consuming. An alternative option allowing rapid and precise prediction of radiosensitivity is necessary.
Methods:
In this study, we exposed 4 in vitro cultured cell lines to various doses of X-rays or carbon ions and then measured their radiosensitivities with a routine colony-forming assay, and monitored the kinetics of cell cycle distribution with routine propidium iodine staining and flow cytometry.
Results:
Based on the results, we correlated cellular radiosensitivity with a dynamic assay of cell cycle distribution, specifically, the negative correlation of cellular radiosensitivity with the accumulated G2/M arrested cells at 48 hours after exposure. The higher the proportion of accumulated G2/M arrested cells at 48 hours after exposure, the lower the radiosensitivity of the cell line, that is, the higher radioresistance of the cell line.
Conclusion:
These findings provide an optional application of regular cell cycle analysis for the prediction of tumor radiosensitivity.
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