Ziziphus spina-christi leaf extract attenuates mercury chloride-induced testicular dysfunction in rats

Mercury (Hg) is a heavy metal toxicant, causing several adverse reactions to animals and humans including reproductive dysfunction. The potential protective role of Ziziphus spina-christi leaf extract (ZSCLE) against testicular impairments associated with mercury chloride (HgCl₂) exposure in rats was investigated in the current study. Four experimental groups were employed as follows (n = 7): group I served as control, group II was gavaged with ZSCLE (300 mg/kg), group III was administered with HgCl₂ (0.4 mg/kg), and group IV was preadministered with ZSCLE 1 h before HgCl₂. All groups were treated daily for 28 days. The exposure to HgCl₂ caused a marked increase in Hg concentration in the testicular tissue, which was accompanied with a decrease in testis index. A reproductive impairment was recorded following HgCl₂ exposure as verified through the decrease in levels of testosterone, luteinizing, and follicle-stimulating hormones. HgCl₂ was found to enhance the development of oxidative damage in the testicular tissue as presented by the imbalance between pro-oxidants and antioxidant molecules. In addition, excessive release of tumor necrosis factor-α and interleukin-1β was recorded in response to HgCl₂ intoxication. Furthermore, a disturbance in the apoptotic proteins in favor of the pro-apoptotic proteins was also observed following HgCl₂ intoxication. However, ZSCLE administration along with HgCl₂ abolished significantly the molecular, biochemical, and histopathological alterations induced by HgCl₂ intoxication. Our findings suggest that ZSCLE could be used to mitigate reproductive dysfunction associated with HgCl₂ exposure.