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MicroCT vascular network analysis program: Development, validation, and comparison to manufacturer software.

John Peters1,2, Luke Vest1,2, Matthew Schuelke3

  • 1Department of Orthopaedic Surgery, Saint Louis University, St Louis, Missouri.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
|December 17, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed a new, accessible vascular network analysis (VNA) protocol for microcomputed tomography (µCT) scans. This VNA method accurately measures bone vascular networks, outperforming traditional trabecular analysis software, especially in complex bone structures.

Keywords:
MicroCTangiogenesisbone repairsoftwarevascular networks

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Area of Science:

  • Orthopedics
  • Biomedical Engineering
  • Medical Imaging

Background:

  • Bone repair relies on angiogenesis, necessitating precise vascular network measurement.
  • Microcomputed tomography (µCT) offers superior 3D vascular network visualization compared to histology.
  • Existing 3D vascular analysis software can be prohibitively expensive for many labs.

Purpose of the Study:

  • To develop an accessible vascular network analysis protocol using freely available software.
  • To compare the performance of the developed protocol against a commercial µCT trabecular analysis software.

Main Methods:

  • Developed a vascular network analysis (VNA) protocol using Fiji ImageJ and MATLAB.
  • Validated the VNA protocol using in silico models of varying complexity and homogeneity.
  • Compared VNA performance against a µCT trabecular analysis program (MicroCT).

Main Results:

  • VNA demonstrated acceptable percent error (<10%) for most vascular network measurements.
  • VNA and MicroCT showed similar performance for volume but differed significantly in average vessel diameter accuracy.
  • VNA significantly outperformed MicroCT in accurately measuring average vessel diameter in heterogeneous vascular models.

Conclusions:

  • A novel, accessible VNA protocol for analyzing µCT scans of vascular networks has been established.
  • The developed VNA protocol is insensitive to network homogeneity and offers higher accuracy than µCT trabecular analysis software.
  • This accessible VNA method is valuable for orthopedic research requiring precise bone vascular network quantification.