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Updated: Jan 1, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
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MicroRNA-296 functions as a tumor suppressor in breast cancer by targeting FGFR1 and regulating the Wnt/β-catenin

W-M Sun1, W Tao, J-C Li

  • 1Department of General Surgery Breast Ward, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China. miaoyejz@126.com.

European Review for Medical and Pharmacological Sciences
|December 17, 2019
PubMed
Abstract

Insights

This study reveals that miR-296 is significantly decreased in breast cancer (BC) and acts as a tumor suppressor. Restoring miR-296 inhibits BC cell proliferation, invasion, and migration, offering potential therapeutic strategies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Breast cancer (BC) is a prevalent global malignancy with incompletely understood progression mechanisms.
  • MicroRNAs (miRNAs) are recognized for their critical roles in tumorigenesis.
  • The specific role of miR-296 in BC development and progression requires further elucidation.

Purpose of the Study:

  • To investigate the expression levels of miR-296 in breast cancer tissues and cell lines.
  • To determine the functional impact of miR-296 on BC cell proliferation, invasion, and migration.
  • To explore the underlying molecular mechanisms by which miR-296 influences BC progression.

Main Methods:

  • Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) for miR-296 expression analysis.
  • In vitro functional assays (MTT, Transwell) to assess proliferation, invasion, and migration.
  • Immunohistochemistry (IHC), Western blot, and Luciferase reporter assays to investigate molecular targets and pathways (FGFR1, Wnt/β-catenin, EMT).

Main Results:

  • miR-296 expression was significantly downregulated in BC tissues and cell lines compared to normal controls.
  • Reduced miR-296 levels correlated with advanced malignant phenotypes and poorer patient prognosis.
  • Restoration of miR-296 suppressed BC cell proliferation, invasion, and migration in vitro and inhibited tumor growth in vivo.
  • miR-296 functions by regulating Fibroblast Growth Factor Receptor 1 (FGFR1), the Wnt/β-catenin signaling pathway, and Epithelial-Mesenchymal Transition (EMT).

Conclusions:

  • miR-296 exhibits significant anti-tumor functions in breast cancer.
  • The findings suggest miR-296 as a potential biomarker for BC prognosis and a novel therapeutic target for BC treatment.

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