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Updated: Jan 1, 2026
Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity
Published on: February 24, 2017
Long non-coding RNA DANCR induces chondrogenesis by regulating the miR-1275/MMP-13 axis in synovial fluid-derived
1Department of Orthopedics, Jinling Hospital, Nanjing University, School of Medicine, Nanjing, China. LeiZHANG1987MD@163.com.
Objective:
The aim of this study was to examine the role of lncRNA-differentiation antagonizing non-protein coding RNA (DANCR) and its underlying mechanisms in chondrogenesis, more specifically in synovial fluid-derived mesenchymal stem cell (SFMSCs).
Materials And Methods:
The expression levels of DANCR in SFMSCs were measured by qRT-PCR. Luciferase reporter assay and RIP assay were used to investigate the direct target of DANCR and miR-1275 in SFMSCs. The expression of matrix metallopeptidase 13 (MMP13, also known as chondrogenic marker) protein was examined by Western blot. Cell proliferation was analyzed by Cell Counting Kit-8 (CCK-8) assay, while chondrogenic differentiation was explored by sGAG assay.
Results:
Our data indicated that DANCR can promote SFMSCs proliferation and chondrogenesis. In addition, miR-1275 was indicated as a direct target of DANCR. MiR-1275 was negatively regulated by DANCR via competing endogenous RNA (ceRNA) mechanism. Moreover, our data revealed that miR-1275 could bind to MMP13 and regulate its expression.
Conclusions:
Our findings suggested that DANCR was involved in SFMSCs proliferation and chondrogenesis. Mechanistically, DANCR functions as a sponge RNA for miR-1275 that regulates the expression of target gene MMP13. These data provide a therapeutic option for Osteoarthritis (OA).
Insights
Long non-coding RNA DANCR promotes synovial fluid-derived mesenchymal stem cell proliferation and chondrogenesis. DANCR acts as a sponge for miR-1275, regulating matrix metallopeptidase 13 expression, offering a potential therapeutic strategy for Osteoarthritis.
Area of Science:
- Stem cell biology
- Molecular mechanisms of chondrogenesis
- Non-coding RNA research
Background:
- Mesenchymal stem cells (MSCs) are crucial for tissue regeneration.
- Chondrogenesis, the process of cartilage formation, is vital for joint health.
- Dysregulation of chondrogenesis contributes to Osteoarthritis (OA).
Purpose of the Study:
- To investigate the role of long non-coding RNA differentiation antagonizing non-protein coding RNA (lncRNA DANCR) in chondrogenesis.
- To elucidate the molecular mechanisms by which DANCR influences synovial fluid-derived mesenchymal stem cells (SFMSCs).
- To explore the therapeutic potential of DANCR in OA.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) for gene expression analysis.
- Luciferase reporter and RNA immunoprecipitation (RIP) assays to identify direct targets.
- Western blot for protein expression (MMP13).
- Cell Counting Kit-8 (CCK-8) and sGAG assays for proliferation and differentiation assessment.
Main Results:
- DANCR expression was measured in SFMSCs.
- DANCR was found to promote SFMSC proliferation and chondrogenesis.
- MiR-1275 was identified as a direct target of DANCR, regulated via a competing endogenous RNA (ceRNA) mechanism.
- MiR-1275 directly binds to and regulates the expression of matrix metallopeptidase 13 (MMP13).
Conclusions:
- DANCR plays a significant role in SFMSC proliferation and chondrogenesis.
- The mechanism involves DANCR acting as a sponge for miR-1275, which in turn regulates MMP13 expression.
- These findings suggest DANCR as a potential therapeutic target for Osteoarthritis.
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