Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.8K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Systematic characterisation of site-specific proline hydroxylation using hydrophilic interaction chromatography and mass spectrometry.

eLife·2026
Same author

PHD1-dependent hydroxylation of RepoMan (CDCA2) on P604 modulates the control of mitotic progression.

eLife·2026
Same author

Hybrid spectral-spatial domain registration for nanometric tracking in digital in-line holographic microscopy.

Optics letters·2026
Same author

Speckle suppression in digital in-line holographic microscopy through liquid crystal dynamic scattering.

Optics letters·2025
Same author

Open-source sub-nanometer stabilization system for super-resolution fluorescence microscopy.

Light, science & applications·2025
Same author

Rolling into the genome: linking mutations to cellular structure through label-free holographic cytometry.

Light, science & applications·2025

Related Experiment Video

Updated: Jan 1, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.5K

Numerically Enhanced Stimulated Emission Depletion Microscopy with Adaptive Optics for Deep-Tissue Super-Resolved

Piotr Zdańkowski1,2,3, Maciej Trusiak3, David McGloin2,4

  • 1Centre for Gene Regulation and Expression, School of Life Sciences , University of Dundee , Dundee DD1 5EH , United Kingdom.

ACS Nano
|December 17, 2019
PubMed
Summary

We developed a custom STED nanoscopy system with aberration correction and BM3D denoising. This improves 3D super-resolution imaging through thick, aberrating tissues, enhancing effective resolution by 31%.

Keywords:
STED microscopyaberration correctionadaptive opticsdeep imagingfluorescence microscopysuper-resolution microscopy

More Related Videos

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy
10:00

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy

Published on: March 24, 2014

78.4K
Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

20.3K

Related Experiment Videos

Last Updated: Jan 1, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.5K
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy
10:00

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy

Published on: March 24, 2014

78.4K
Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

20.3K

Area of Science:

  • Microscopy
  • Biophysics
  • Optical Engineering

Background:

  • Stimulated Emission Depletion (STED) nanoscopy faces challenges with signal-to-noise ratio due to photobleaching and optical aberrations.
  • High-power depletion lasers in STED increase photodamage risk, and the depletion beam is sensitive to aberrations.

Purpose of the Study:

  • To demonstrate a custom-built STED microscope with automated aberration correction for 3D super-resolution imaging.
  • To investigate the Block-Matching and 3D Collaborative Filtering (BM3D) method for image denoising and enhancement in STED nanoscopy.
  • To validate the combined approach for imaging through thick, aberrating biological samples.

Main Methods:

  • Development of a custom STED microscope with integrated automated aberration correction.
  • Application of the Block-Matching and 3D Collaborative Filtering (BM3D) algorithm for numerical image denoising.
  • Fourier Ring Correlation (FRC) metric for quantifying resolution enhancement.
  • Experimental validation using 3D super-resolved imaging of neuronal axons in induced pluripotent stem cells.

Main Results:

  • Achieved 3D super-resolution imaging through 80 μm thick, highly aberrating tissue.
  • Demonstrated a 31% increase in effective resolution using BM3D denoising.
  • Obtained lateral and axial resolutions of 204 nm and 310 nm, respectively.
  • Successfully imaged axons in differentiated induced pluripotent stem cells.

Conclusions:

  • Automated aberration correction and BM3D denoising significantly enhance STED nanoscopy performance.
  • The combined approach enables high-quality 3D super-resolution imaging in challenging biological samples.
  • This technique offers improved effective resolution and reduced noise for detailed cellular structure visualization.