Comparative lipid profile of four edible shellfishes by UPLC-Triple TOF-MS/MS

An ultra performance liquid chromatography-Triple time of flight mass spectrometry (UPLC-Triple TOF-MS/MS) method were established to characterize the lipid profiles in four shellfish species. More than 600 lipid molecular species belonging to 14 classes were detected. Phospholipids (PLs) were predominant in Chlamys farreri (54.9%) and glycerolipids (GLs) were dominant in Ostrea gigas (51.6%). PLs that contained polyunsaturated fatty acids (PUFAs) such as PC (16:0/20:5), PC (16:0/22:6) and PE (18:0/22:6) were the main molecular species. Especially, the percentage of sphingolipids (SLs) in four shellfishes is considerable (18.8-38.6%), the characterization of their special long-chain base (LCB) structure (mainly d19:3) and N-acyl group (mainly 16:0) was realized. Several SL subclasses with low abundance in four shellfish species, such as ceramide 2-aminoethylphosphonate (CAEP) and deoxy-ceramide (DeoxyCer), were also detected. These active lipids identified by this method have potential value in revealing the nutritional value of shellfishes and serving as biomarkers for distinguishing different shellfishes.