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Subcellular distribution of newly synthesized glycerolipids in intact and homogenized rat adipocytes
1Département de Biochimie médicale, Université de Genève, Suisse.
Abstract:
1. Homogenization of rat adipocytes was found to inhibit the transfer of newly synthesized phospholipids and glycerides from the membranes to the storage lipids. 2. In intact adipocytes, only 23 +/- 3.4% of the phospholipids and 0.44 +/- 0.12% of the glycerides remained bound to the membranes, while for the homogenate, the comparable values obtained were 74 +/- 5.2% and 90 +/- 0.3% respectively. 3. Various factors that might be involved in the liberation of the glycerolipids from the adipocyte esterification sites are discussed.
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