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Updated: Jan 1, 2026
A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis
Published on: August 12, 2020
B lymphocytopenia and Bregs in a not-to-die murine sepsis model
Kensuke Umakoshi1, Mohammed E Choudhury2, Ryutaro Nishioka2
1Department of Emergency and Critical Medicine, Graduate School of Medicine, Ehime University, Toon, Ehime, Japan; Advanced Emergency and Critical Care Center, Ehime Prefectural Central Hospital, Matsuyama, Ehime, Japan.
Abstract:
Sepsis is a leading cause of mortality in intensive care units due to multi-organ failure caused by dysregulated immune reactions. In this study, kinetic changes in the immune system were analyzed for 72 h in cecal ligation and puncture (CLP)-induced septic mice while preventing animal death by keeping body temperature. Increase of myeloid cells and decrease of B cells in circulation at 6 h after CLP were markedly observed. At the same time point, interleukin (IL)-10 expressing CD5+ regulatory B cells (Bregs) appeared. IL-10 and programmed death-ligand 1 (PD-L1) mRNA as well as IL-1β, IL-6 and interferon γ (IFNγ) mRNA was increased in the spleen at 6 h. A gradual decrease in Bcl-2 and abrupt increase of Bim expression in the spleen at the late phase were also found. These results showed that B lymphocytopenia with the appearance of Bregs is the earliest event, likely leading to immunoparalysis in sepsis.
