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Long Noncoding RNA MNX1 antisense RNA 1 Exerts Oncogenic Functions in Bladder Cancer by Regulating miR-218-5p/RAB1A

Jun Wang1, Huiwu Xing1, Abdul Aziz Nikzad1

  • 1Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.

The Journal of Pharmacology and Experimental Therapeutics
|December 18, 2019
PubMed

Insights

Long noncoding RNA MNX1-AS1 promotes bladder cancer progression by regulating RAB1A expression via sponging miR-218-5p. Reduced MNX1-AS1 inhibits proliferation, migration, invasion, and metastasis, suggesting it as a therapeutic target and biomarker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNA MNX1 antisense RNA 1 (MNX1-AS1) is overexpressed in bladder cancer.
  • The precise molecular mechanisms of MNX1-AS1 in bladder cancer remain unclear.

Purpose of the Study:

  • To elucidate the role and molecular mechanism of MNX1-AS1 in bladder cancer progression.
  • To investigate MNX1-AS1 as a potential therapeutic target and biomarker for bladder cancer.

Main Methods:

  • Gain- and loss-of-function experiments were performed in bladder cancer cell lines.
  • Mechanistic studies involved assessing the interaction between MNX1-AS1, miR-218-5p, and RAB1A.
  • In vivo experiments using mouse models were conducted to evaluate tumor growth and metastasis.

Main Results:

  • Reduced MNX1-AS1 expression suppressed bladder cancer cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT).
  • Overexpression of MNX1-AS1 exhibited opposite effects, promoting these malignant phenotypes.
  • MNX1-AS1 acts as a molecular sponge for miR-218-5p, thereby regulating RAB1A expression.
  • In vivo, decreased MNX1-AS1 expression inhibited tumor growth and metastasis.

Conclusions:

  • MNX1-AS1 promotes bladder cancer initiation and progression by modulating the miR-218-5p/RAB1A axis.
  • MNX1-AS1 may serve as a novel therapeutic target and a potential biomarker for bladder cancer metastasis and prognosis.

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