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Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
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Practical Applications of Biofluorescent Particle Counting in Environmental Monitoring Investigations.

Aditya Prasad1, Annett Milling2, James Hauschild3

  • 1AstraZeneca, Philadelphia, PA, USA.

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|December 18, 2019
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Summary

Biofluorescent particle counting (BFPC) offers real-time detection of microorganisms, significantly speeding up environmental monitoring (EM) investigations. This method aids in identifying root causes and implementing corrective actions faster than traditional culture-based techniques.

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Area of Science:

  • Microbiology
  • Environmental Science
  • Industrial Microbiology

Background:

  • Environmental monitoring (EM) investigations for excursions are often lengthy.
  • Traditional culture-based methods cause delays in identifying root causes and implementing corrective actions.
  • There is a need for faster, real-time microbial detection in EM.

Purpose of the Study:

  • To introduce biofluorescent particle counting (BFPC) as a tool to expedite EM investigations.
  • To demonstrate BFPC's utility in real-time microbial detection and risk reduction.
  • To provide guidance and case studies on implementing BFPC in industrial settings.

Main Methods:

  • Utilizing biofluorescent particle counting (BFPC) for rapid, real-time detection of viable microorganisms in air samples.
  • Supplementing routine environmental monitoring (EM) with BFPC for enhanced investigation capabilities.
  • Analyzing a case study of a mold excursion using BFPC for root cause analysis.

Main Results:

  • BFPC significantly reduces investigation timelines compared to culture-based methods.
  • Real-time detection enables immediate identification of microbial contamination sources.
  • BFPC supports faster root cause analysis and the implementation of effective remedial actions.

Conclusions:

  • Biofluorescent particle counting (BFPC) is an effective risk-reduction tool for environmental monitoring (EM).
  • Implementing BFPC accelerates investigations, leading to quicker corrective and preventative actions.
  • Companies can achieve significant business benefits through the adoption of BFPC technology.