Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The 2025 Westlake Autumn Symposium for Al Proteomics and Virtual Cell.

Genomics, proteomics & bioinformatics·2026

Same author

Unveiling proteomic and peptide-level modifications in cerebrospinal fluid and plasma in normal cognitive aging.

Communications medicine·2025

Same author

A Flexible End-to-End Automated Sample Preparation Workflow Enables Standardized and Scalable Bottom-up Proteomics.

Analytical chemistry·2025

Same author

Enhancing Sensitivity in Low-Load Proteomics Orbitrap Workflows via SLIM Integration.

Analytical chemistry·2025

Same author

A tissue-specific atlas of protein-protein associations enables prioritization of candidate disease genes.

Nature biotechnology·2025

Same author

Understanding the molecular diversity of synapses.

Nature reviews. Neuroscience·2024

Same journal

PCSK5 promotes angiogenesis and cardiac repair after myocardial infarction.

Nature communications·2026

Same journal

PfApiAT2 is a proline transporter essential for the transmission of Plasmodium falciparum by the mosquito vector.

Nature communications·2026

Same journal

Transient distortions of the South Atlantic Anomaly radiation environments driven by electric fields.

Nature communications·2026

Same journal

Structural basis of the regulation by CDK11 kinase of early spliceosome activation and evidence for its proofreading by DHX15 helicase.

Nature communications·2026

Same journal

Structural and mechanistic insights into primer synthesis initiation by DNA primase.

Nature communications·2026

Same journal

Changes in heritability and shared environmentality of educational attainment across twentieth-century Norway.

Nature communications·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 1, 2026

Flow Cytometric Characterization of Murine B Cell Development

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

Classification of mouse B cell types using surfaceome proteotype maps.

Marc van Oostrum^1,2,3,4, Maik Müller^1,2,3, Fabian Klein⁵

¹Biomedical Proteomics Platform, Department of Health Sciences and Technology, ETH Zurich, 8093, Zurich, Switzerland.

Nature Communications

|December 18, 2019

Summary

This summary is machine-generated.

We developed autoCSC, an automated method for mapping cell surface proteins, even with limited samples. This technology reveals new details about developing mouse B cell populations.

More Related Videos

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

Related Experiment Videos

Last Updated: Jan 1, 2026

Flow Cytometric Characterization of Murine B Cell Development

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

Area of Science:

Proteomics
Glycomics
Immunology

Background:

Cell surface proteotype and extracellular glycosylation site identification is difficult with limited sample sizes.
Existing Cell Surface Capture (CSC) technology faces challenges in sensitivity, reproducibility, and throughput.

Purpose of the Study:

To miniaturize and automate the Cell Surface Capture (CSC) technology for enhanced performance.
To create population-specific surfaceome maps of developing mouse B cells.
To identify novel developmental cell subpopulations using targeted flow cytometry.

Main Methods:

Miniaturization and automation of the Cell Surface Capture (CSC) technology, termed autoCSC.
Application of autoCSC to generate surfaceome maps of developing mouse B cells.
Utilizing targeted flow cytometry for subpopulation identification.

Main Results:

autoCSC significantly increases sensitivity, reproducibility, and throughput compared to the original CSC method.
Population-specific surfaceome maps of developing mouse B cells were successfully generated.
Targeted flow cytometry identified previously unknown developmental cell subpopulations.

Conclusions:

The automated CSC (autoCSC) technology provides a more sensitive and efficient approach for cell surface proteotype analysis.
autoCSC enables detailed mapping of cell surfaces, aiding in the discovery of cellular heterogeneity.
This technology advances the study of cell development and subpopulation identification in systems biology.