Amino terminal sequence of hog intrinsic factor

Area of Science:

• Biochemistry
• Proteomics

Background:

• Intrinsic factor is crucial for vitamin B12 absorption.
• Understanding the structure of intrinsic factor aids in diagnosing related deficiencies.

Purpose of the Study:

• To purify hog intrinsic factor.
• To determine the N-terminal amino acid sequence of hog intrinsic factor.
• To compare the sequence with human intrinsic factor.

Main Methods:

• Purification of hog intrinsic factor using labile ligand affinity chromatography.
• N-terminal amino acid sequencing.

Main Results:

• Hog intrinsic factor was successfully purified from gastric mucosa.
• The N-terminal amino acid sequence (Thr-Arg-Ser-Ser-Cys-Ser-Val-Pro-Ser-Ala-Glu-Gln-Pro-Leu-Val-Asn-Gly-Ile-Gln-Val-Leu-Met) was determined.
• Approximately 60% of the preparation lacked the first two residues.
• A high degree of homology was observed between the N-terminal sequences of hog and human intrinsic factor.

Conclusions:

• The purification and sequencing of hog intrinsic factor provide valuable data for comparative analysis.
• The homology suggests conserved structural and functional roles between species.