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Published on: October 12, 2017
Novel Associations between Related Proteins and Cellular Effects of High-Density Lipoprotein
Seungbum Choi1, Yae Eun Park2,3, Eun Jeong Cheon1
1Cardiovascular Research Institute, Yonsei University College of Medicine, Seoul, Korea.
Insights
High-density lipoprotein (HDL) function is linked to specific proteins. This study identified and ranked HDL proteins associated with cholesterol efflux capacity, revealing new insights into HDL functionality.
Area of Science:
- Cardiovascular Biology
- Proteomics
- Lipid Metabolism
Background:
- High-density lipoprotein (HDL) plays a crucial role in reverse cholesterol transport.
- Understanding the structure-function relationship of HDL is vital for cardiovascular health.
- HDL-associated proteins significantly influence HDL's biological functions.
Purpose of the Study:
- To identify and rank proteins associated with high-density lipoprotein (HDL).
- To investigate the relationship between HDL-associated proteins and key HDL functions.
- To determine proteins linked to cholesterol efflux capacity and other HDL functionalities.
Main Methods:
- Analysis of HDLs isolated from 48 participants.
- Assessment of cholesterol efflux capacity, HDL's effect on nitric oxide production, and vascular cell adhesion molecule-1 expression.
- Quantification of protein relative abundance using normalized spectral abundance factor ratio.
Main Results:
- Six proteins (thyroxine-binding globulin, alpha-1B-glycoprotein, plasma serine protease inhibitor, vitronectin, angiotensinogen, serum amyloid A-4) were more abundant in HDLs with high cholesterol efflux capacity.
- Three proteins (complement C4-A, alpha-2-macroglobulin, immunoglobulin mu chain C region) were less abundant in HDLs with high cholesterol efflux capacity.
- No significant protein abundance changes were observed for nitric oxide production or VCAM-1 expression.
Conclusions:
- This study successfully ranked HDL-associated proteins based on their abundance in HDLs with high functional capacities.
- Multiple novel proteins linked to cholesterol efflux capacity were identified.
- The findings contribute to a deeper understanding of HDL protein composition and its functional implications.
Background And Objectives:
Recent studies have examined the structure-function relationship of high-density lipoprotein (HDL). This study aimed to identify and rank HDL-associated proteins involved in several biological function of HDL.
Methods:
HDLs isolated from 48 participants were analyzed. Cholesterol efflux capacity, effect of HDL on nitric oxide production, and vascular cell adhesion molecule-1 expression were assessed. The relative abundance of identified proteins in the highest vs. lowest quartile was expressed using the normalized spectral abundance factor ratio.
Results:
After adjustment by multiple testing, six proteins, thyroxine-binding globulin, alpha-1B-glycoprotein, plasma serine protease inhibitor, vitronectin, angiotensinogen, and serum amyloid A-4, were more abundant (relative abundance ratio ≥2) in HDLs with the highest cholesterol efflux capacity. In contrast, three proteins, complement C4-A, alpha-2-macroglobulin, and immunoglobulin mu chain C region, were less abundant (relative abundance ratio <0.5). In terms of nitric oxide production and vascular cell adhesion molecule-1 expression, no proteins showed abundance ratios ≥2 or <0.5 after adjustment. Proteins correlated with the functional parameters of HDL belonged to diverse biological categories.
Conclusions:
In summary, this study ranked proteins showing higher or lower abundance in HDLs with high functional capacities and newly identified multiple proteins linked to cholesterol efflux capacity.
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