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Updated: Jan 1, 2026

Bergmeyer Glucose Quantification for Microbiological Samples
Published on: January 17, 2025
Highly sensitive and selective non-enzymatic glucose detection based on indigo carmine/hemin/H2O2 chemiluminescence
Tadesse Haile Fereja1, Shimeles Addisu Kitte, Muhammad Nadeem Zafar
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin 130022, P.R. China. unihanshuang@163.com mirandazhang@ciac.ac.cn guobaoxu@ciac.ac.cn.
Abstract:
A new chemiluminescence (CL) system, indigo carmine/glucose/hemin/H2O2, has been found and developed for non-enzymatic detection of indigo carmine (IC) and glucose. The CL response increases linearly with IC concentrations from 3.2 μM to 10 mM and glucose concentrations from 0.06 μM to 3.5 mM. The detection limits are 1.45 μM and 15.0 nM for IC and glucose, respectively. This method allows the determination of glucose in blood and urine after simple dilution. The recoveries for the determination of glucose are between 98.5% and 101.0% in blood and between 98.5% and 101.3% in urine. This method shows good sensitivity, selectivity, simplicity, and is low cost, suggesting its promising broad applications.
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