Related Experiment Video
Updated: Jan 1, 2026
07:03
Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells
Published on: September 23, 2022
2.5K
Human Pluripotent Stem Cell-Derived Extracellular Vesicles: Characteristics and Applications
Richard Jeske1, Julie Bejoy1, Mark Marzano1
1Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University, Tallahassee, Florida.
Tissue Engineering. Part B, Reviews
|December 19, 2019
Summary
Human induced pluripotent stem cell-derived extracellular vesicles (EVs) show promise for treating neurological diseases. These EVs facilitate cell-cell communication and can be engineered for targeted drug delivery, offering new therapeutic avenues.
Area of Science:
- Biomedical Sciences
- Regenerative Medicine
- Cell Biology
Background:
- Extracellular vesicles (EVs), including exosomes and microvesicles, are crucial for biological processes and tissue homeostasis.
- Stem cell-derived EVs offer protective effects, reducing oxidative stress and apoptosis in recipient cells.
- EVs serve as biomarkers and are promising for drug delivery due to their ability to cross the blood-brain barrier.
Purpose of the Study:
- To review the properties of human induced pluripotent stem cell (iPSC)-derived EVs.
- To summarize EV biogenesis, composition, purification, and characterization methods.
- To discuss applications of iPSC-derived EVs in neurological diseases.
Main Methods:
- Literature review of studies on iPSC-derived EVs.
- Summary of EV biogenesis and composition.
- Analysis of purification and characterization techniques.
Main Results:
- iPSC-derived EVs exhibit potential for therapeutic applications.
- EVs derived from iPSCs of various lineage specifications have distinct properties.
- Applications in neurological diseases highlight the role of cell-cell interactions.
Conclusions:
- iPSC-derived EVs are significant for cell-free therapies.
- These EVs play a key role in neural cellular phenotype and neurodegeneration.
- Findings support the development of stem cell-derived cell-free products for therapeutic use.
Related Concept Videos
01:36Overview of Exosomes
3.4K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
3.4K
00:58Embryonic Stem Cells
32.0K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.0K
00:57Embryonic Stem Cells
4.6K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
4.6K
01:06Induced Pluripotent Stem Cells
5.2K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
5.2K