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In-vivo and in-vitro association between leukemic cells and marrow stromal cells: a murine model
1Mary Jeane Price-Jones Kempner Laboratory for Hematology Research, University of Texas Medical Branch, Galveston.
Several reports indicate that marrow stromal cells are conducive to development of both myeloid and lymphoid leukemic cell lines in vitro. We present data on an in-vitro/in-vivo murine model that establishes that: (i) murine LL cell lines (L5178Y, P388 and L1210) invade the bone marrow when injected via the tail vein; (ii) degree of marrow invasion by LL can be determined quantitatively; (iii) there is a close association or adherence between MSC and LL cells in vivo; and (iv) in-vitro adherence of murine LL cells to MSC can be quantitated. This in-vitro/in-vivo murine model will be helpful in deciphering the importance of heterotypic adherence between marrow stromal cells and leukemic cells in vivo.
Several reports indicate that marrow stromal cells are conducive to development of both myeloid and lymphoid leukemic cell lines in vitro. We present data on an in-vitro/in-vivo murine model that establishes that: (i) murine LL cell lines (L5178Y, P388 and L1210) invade the bone marrow when injected via the tail vein; (ii) degree of marrow invasion by LL can be determined quantitatively; (iii) there is a close association or adherence between MSC and LL cells in vivo; and (iv) in-vitro adherence of murine LL cells to MSC can be quantitated. This in-vitro/in-vivo murine model will be helpful in deciphering the importance of heterotypic adherence between marrow stromal cells and leukemic cells in vivo.