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A High-Throughput, Seedling Screen for Plant Immunity.

Alexandre Martel¹, Timothy Lo¹, Darrell Desveaux^1,2

¹Department of Cell and Systems Biology, University of Toronto, Ontario, Canada.

Molecular Plant-Microbe Interactions : MPMI

|December 19, 2019

Summary

We developed a new, cost-effective seedling assay for high-throughput plant pathology screening. This method efficiently identifies plant immune responses to pathogens, aiding in the discovery of new plant immunity genes.

Keywords:

Arabidopsis thaliana Pseudomonas syringae avirulence factors effector-triggered immunity (ETI)effectors resistance genes screen seedling type III secreted effector (T3SE)type-3 secretion

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Area of Science:

Plant Pathology
Molecular Plant-Microbe Interactions
Genetics

Background:

Understanding plant-microbe interactions is crucial for plant immunity.
High-throughput in vivo pathology screens are limited.
Technological advances facilitate molecular analysis but not whole-organism screening.

Purpose of the Study:

To develop a high-throughput, nondestructive seedling pathology assay.
To apply the assay for identifying Arabidopsis thaliana effector-triggered immunity (ETI) responses.
To enable quantitative differentiation of plant genotypes and host immunity gene discovery.

Main Methods:

A 48-well microplate-based, nondestructive seedling assay with spray inoculation.
Semiautomated, quantitative recording of disease symptoms.
Application to Arabidopsis thaliana and Pseudomonas syringae interactions.

Main Results:

The assay successfully recapitulated known ETI responses in Arabidopsis.
Quantitative differentiation of responses across diverse plant genotypes was achieved.
The assay's results were used for genome-wide association studies to identify host immunity genes.

Conclusions:

A novel, cost-effective, and high-throughput seedling pathology assay was established.
The assay facilitates efficient screening of plant immune responses and genetic variation.
This method aids in identifying host immunity genes and understanding plant-microbe interactions.