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Related Concept Videos

Enzyme Kinetics01:19

Enzyme Kinetics

103.4K
Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
103.4K

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Fluorescence detection methods for microfluidic droplet platforms
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Droplet-based optofluidic systems for measuring enzyme kinetics.

David Hess1, Tianjin Yang1,2, Stavros Stavrakis3

  • 1Institute for Chemical and Bioengineering, ETH Zürich, Vladimir Prelog Weg 1, 8093, Zürich, Switzerland.

Analytical and Bioanalytical Chemistry
|December 20, 2019
PubMed
Summary

Microfluidic systems offer a high-throughput, sensitive method for analyzing enzyme kinetics. This review introduces novel tools and detection methods for efficient enzyme kinetic studies, overcoming limitations of conventional techniques.

Keywords:
Droplet-based microfluidicsEnzyme kineticsFluorescence detectionLabel-free detectionOptofluidics

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Area of Science:

  • Biochemistry
  • Chemical Engineering
  • Biotechnology

Background:

  • Enzyme kinetics are crucial for understanding cellular metabolism and industrial enzyme applications.
  • Conventional enzyme kinetic analysis is often laborious, time-consuming, and costly.
  • Droplet-based microfluidics offers advantages like minimal sample consumption, high throughput, and efficient transfer.

Purpose of the Study:

  • To review novel microfluidic tools and detection methods for high-throughput, sensitive enzyme kinetics analysis.
  • To introduce basic enzyme kinetics concepts and microfluidic approaches, focusing on segmented flow.
  • To critically assess current platforms for enzyme kinetics measurement and suggest improvements.

Main Methods:

  • Review of enzyme kinetics principles.
  • Description of microfluidic techniques, particularly segmented flow.
  • Analysis of state-of-the-art platforms for high-throughput enzyme kinetics measurement.

Main Results:

  • Microfluidics, especially segmented flow, provides precise control, efficient transfer, multiplexing, and integration for enzyme kinetics.
  • Current platforms for high-throughput enzyme kinetics analysis are categorized and critically evaluated.
  • Strategies for enhancing measurements in droplet-based microfluidics are proposed.

Conclusions:

  • Microfluidic systems represent a significant advancement for enzyme kinetics research.
  • Novel tools and detection methods enable more efficient and sensitive analysis.
  • Addressing limitations in current droplet-based microfluidic approaches will further advance the field.