Temperature Responsive Shape-Memory Scaffolds with Circumferentially Aligned Nanofibers for Guiding Smooth Muscle Cell Behavior

Structural simulation of the smooth muscle layer plays an important role in tissue engineering of blood vessels for the replacement of damaged arteries. However, it is difficult to construct small-diameter tubular scaffolds to homogenously locate and align smooth muscle cells (SMCs). In this work, novel temperature responsive shape-memory scaffolds are designed for SMC culturing. The scaffolds are composed of an outer layer of poly(lactide-glycolide-trimethylene carbonate) (PLGATMC) for programming the deformation from planar to small-diameter tubular shape and an inner layer of aligned nanofibrous membrane of poly(lactide-glycolide)/chitosan (PLGA/CS) to regulate cell adhesion, proliferation, and morphology. The SMC behaviors and functions are dependent on the PLGA/CS ratios of membranes, and the scaffold with PLGA/CS 7:3 membrane exhibits the most suitable ability to regulate SMC behavior. The PLGA/CS@PLGATMC scaffold can be deformed into a temporary planar at 20 °C for convenient seeding and attachment of SMCs and then immediately self-rolled into 3D tube at 37 °C. The proposed strategy offers a practical approach for the development of small-diameter vascular scaffolds from 2D planar into 3D tubular shape by self-rolling.