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Related Experiment Video

Updated: Jan 1, 2026

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
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Mapping Enzyme Activity on Tissue by Functional Mass Spectrometry Imaging.

Brett R Hamilton1, David L Marshall2, Nicholas R Casewell3

  • 1Centre for Advanced Imaging, and Centre for Microscopy and Microanalysis, The University of Queensland, Brisbane, QLD, 4072, Australia.

Angewandte Chemie (International Ed. in English)
|December 20, 2019
PubMed
Summary

We developed a new mass spectrometry imaging method to map enzyme activity directly in tissues. This technique visualizes enzymatic function in situ, aiding disease research.

Keywords:
PLA2enzymesfunctional assayslipidsmass spectrometry imaging

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Enzymes are crucial for physiological processes and implicated in diseases.
  • Understanding enzyme activity spatial distribution in tissues is vital for health and disease research.
  • Current methods may lack the resolution or specificity to map enzyme activity in situ.

Purpose of the Study:

  • To present a novel mass spectrometry imaging (MSI) method for assaying spatial distribution of enzymatic activity directly from tissue.
  • To demonstrate the capability of MSI for mapping phospholipase activity and specificity.
  • To establish a generalizable method for in situ imaging of biological activity.

Main Methods:

  • Developed a novel mass spectrometry imaging (MSI) approach.
  • Applied MSI to tissue sections incubated with specific phospholipid substrates.
  • Generated high-resolution spatial maps of enzymatic activity.
  • Compared MSI data with histological images of the same tissue sections.

Main Results:

  • Successfully mapped the spatial distribution of phospholipase activity and specificity in tissue sections.
  • Achieved high-resolution imaging of enzymatic activity directly from tissue.
  • Demonstrated the ability to compare functional MSI data with histological context.

Conclusions:

  • Functional mass spectrometry imaging (MSI) is a novel and generalizable method for imaging enzymatic activity in situ.
  • This technique provides high-resolution maps of enzyme activity, aiding the study of enzymatic functions in health and disease.
  • MSI offers a powerful tool for investigating pathologies linked to enzymatic dysregulation.