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Updated: Jan 1, 2026

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
Published on: December 6, 2018
[Stability of ZVI-dependent Autotrophic Denitrification by ANAMMOX Bacteria]
Wen-Jing Zhang1,2,3, Yong Huang1,2,3, Zhen Bi1,2,3
1National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Suzhou 215009, China.
Abstract:
Fe0-dependent autotrophic denitrification mediated by anaerobic ammonia oxidation (ANAMMOX) bacteria was carried out in continuous flow reactors under non-strict anaerobic conditions. Three strategies, including adjusting the influent pH, adding Fe2+, and regular renewal with fresh iron powder, were used to investigate the appropriate conditions to maintain the long-term stability of this process. The results showed that the nitrate removal efficiency declined due to Fe0 passivation over time, and this ultimately led to the reaction becoming unsustainable. Neither reducing the influent pH (within the pH range 5-7) nor adding a quantity of Fe2+ had an obvious effects on the sustainability of this process. Instead, the nitrate removal efficiency and the sustainability of the reactor was enhanced significantly by regular renewal with fresh Fe0 powder. Compared with the control group (with a 7-day stable operation), the experimental setup was run steadily for at least 60 days, and the average nitrate removal efficiency was increased by 22.23%. Consequently, maintaining an adequate amount of activated Fe0 and eliminating the adverse effects of Fe0 passivation are vital for the sustainable operation of Fe0-dependent autotrophic denitrification as mediated by ANAMMOX bacteria.
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