Related Experiment Video

Updated: Jan 1, 2026

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
07:55

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

Published on: March 7, 2019

8.5K

The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast

Io Nam Wong1, Jacqueline Ps Neo1, Judith Oehler1

  • 1Department of Biochemistry, University of Oxford, Oxford, United Kingdom.

Elife
|December 20, 2019
PubMed

Insights

The DNA helicase Fml1 suppresses genomic deletions caused by inter-fork strand annealing (IFSA). This suppression requires Fml1

Area of Science:

  • Molecular Biology
  • Genetics
  • DNA Replication

Background:

  • Inter-fork strand annealing (IFSA) can cause genomic deletions during DNA replication fork convergence.
  • The FANCM-related DNA helicase Fml1 was previously suggested as a potential suppressor of IFSA.

Purpose of the Study:

  • To confirm Fml1's role in suppressing IFSA.
  • To elucidate the mechanism by which Fml1 suppresses IFSA.

Main Methods:

  • Genetic analysis to confirm Fml1's suppressor function.
  • Biochemical assays to assess Fml1's catalytic activity and interaction with Mhf1-Mhf2.
  • In vitro assays to demonstrate Fml1's role in regressed fork restoration.

Main Results:

  • Fml1 was confirmed to suppress IFSA.
  • Fml1's suppression activity is dependent on its catalytic activity and interaction with Mhf1-Mhf2 via its C-terminal domain.
  • Fml1 was shown to catalyze regressed fork restoration in vitro, providing a mechanism for IFSA suppression.

Conclusions:

  • Fml1 is a key suppressor of IFSA-mediated genomic deletions.
  • Fml1 functions by restoring regressed replication forks, preventing aberrant DNA repair pathways.
  • Understanding Fml1's mechanism provides insights into maintaining genome stability during DNA replication.
Keywords:
DNA replicationRad52S. pombechromosomesgene expressionhomologous recombinationreplication fork barrierreplication fork collapsereplication termination

More Related Videos

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy12:04

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

10.5K
Microscopy of Fission Yeast Sexual Lifecycle07:47

Microscopy of Fission Yeast Sexual Lifecycle

Published on: March 9, 2016

15.1K

Related Concept Videos

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.2K
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
40.2K
The DNA Replication Fork01:02

The DNA Replication Fork

17.9K
Disassembly of Intermediate Filaments01:35

Disassembly of Intermediate Filaments

Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
2.5K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.8K
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.1K