Related Experiment Video

Updated: Jan 1, 2026

Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro
05:47

Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro

Published on: May 10, 2021

4.5K

Heat shock protein 90 is downregulated in calcific aortic valve disease

Jonna Weisell1, Pauli Ohukainen2, Juha Näpänkangas3

  • 1School of Pharmacy, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland.

BMC Cardiovascular Disorders
|December 21, 2019
PubMed

Insights

Researchers identified key protein changes in calcific aortic valve disease (CAVD). Heat shock protein 90 (HSP90) was found to be downregulated, offering potential therapeutic targets for this progressive condition.

Area of Science:

  • Cardiovascular Biology
  • Proteomics
  • Molecular Medicine

Background:

  • Calcific aortic valve disease (CAVD) is an atheroinflammatory condition leading to progressive aortic valve calcification.
  • Current treatment options for CAVD are limited, and its molecular mechanisms are not fully understood.
  • Proteomic analysis offers a promising approach to uncover novel factors involved in CAVD pathogenesis.

Purpose of the Study:

  • To identify novel proteins associated with calcific aortic valve disease (CAVD) through a comprehensive proteomic study.
  • To elucidate the molecular mechanisms underlying the progression of aortic valve stenosis.
  • To provide a foundation for developing effective pharmacological treatments for CAVD.

Main Methods:

  • Comparative proteomic analysis using two-dimensional difference gel electrophoresis (2D-DIGE).
  • Protein identification via mass spectrometry.
  • Validation of key protein expression changes using Western blot and immunohistochemistry.
  • Pathway analysis (Ingenuity Pathway Analysis - IPA) to predict regulatory networks in CAVD.

Main Results:

  • Upregulation of complement 9 (C9), serum amyloid P-component (APCS), and transgelin observed in patients with aortic valve stenosis.
  • Downregulation of heat shock protein 90 (HSP90), protein disulfide isomerase A3 (PDIA3), annexin A2 (ANXA2), and galectin-1 in patients with aortic valve stenosis.
  • Western blot confirmed the decreased protein expression of HSP90.

Conclusions:

  • This study presents a novel dataset of proteomic alterations in calcific aortic valve disease (CAVD).
  • The downregulation of the pro-inflammatory cytosolic protein HSP90 is a significant finding in CAVD.
  • These proteomic insights may pave the way for future therapeutic strategies targeting CAVD.
Abstract

Keywords:
Aortic valve stenosisCalcified aortic valve diseaseHeat-shock proteinProteomics

More Related Videos

Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat04:30

Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat

Published on: February 4, 2021

3.6K
Isolation of Human Primary Valve Cells for In vitro Disease Modeling07:31

Isolation of Human Primary Valve Cells for In vitro Disease Modeling

Published on: April 16, 2021

3.1K

Related Concept Videos

Aortic Regurgitation III: Medical Management01:25

Aortic Regurgitation III: Medical Management

Aortic regurgitation (AR) is when the aortic valve does not close or seal properly, leading to backward blood circulation from the aorta into the left ventricle during diastole. Common causes of AR include rheumatic heart disease, congenital valve defects, and aortic root dilation. Managing AR requires a multifaceted approach to alleviate symptoms, preserve left ventricular function, and address the underlying cause of the regurgitation. Patients with symptomatic AR or significant left...
326
Synthesis and Functions of Calcitonin00:51

Synthesis and Functions of Calcitonin

Calcitonin, a vital polypeptide hormone, regulates calcium levels within body fluids. It is released by the parafollicular cells, also known as C cells, situated in the follicular epithelium of the thyroid gland. Calcitonin responds to fluctuations in blood calcium levels and the influence of gastrointestinal hormones like gastrin and cholecystokinin.
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...
4.0K
Aortic Regurgitation I: Introduction01:15

Aortic Regurgitation I: Introduction

IntroductionAortic regurgitation is characterized by the backward flow of blood from the aorta into the left ventricle during diastole and arises from the improper closure of the aortic valve. This condition results in left ventricular volume overload and can stem from both acute and chronic etiologies, each contributing uniquely to the disease's progression and symptomatology.Acute and Chronic CausesAcute aortic regurgitation often results from events that suddenly impair the integrity of the...
395
Aortic Regurgitation II: Clinical Features and Diagnostic Tests01:22

Aortic Regurgitation II: Clinical Features and Diagnostic Tests

Aortic valve regurgitation (AR) occurs when the aortic valve fails to close properly, allowing blood to flow backward from the aorta into the left ventricle. This backflow can result in two distinct clinical presentations: acute and chronic AR, each characterized by its own set of symptoms and physical findings.Acute Aortic RegurgitationAcute AR presents with a sudden onset of severe symptoms. Patients typically experience profound dyspnea (shortness of breath), chest pain, and signs of left...
333