Optimization and characterization of oligosaccharides production from citrus peel waste resource using Aspergillus...

Oligosaccharides have many growth-promoting properties for crops and are effective for fighting off various diseases in agriculture. Producing oligosaccharides from waste fruit peel by using food microorganisms will be a potential approach to provide the high-value products for sustainable development of green agriculture. Aspergillus niger 1805 was isolated from citrus peel and identified by internal transcribed spacer (ITS1-ITS4) sequencing. A. niger 1805 grew well only with waste citrus peel (WCP) as the sole medium. >50% WCP was degraded into oligosaccharides by fermentation with A. niger at 37 °C, pH 5.0 and 4 mM Ca²⁺ within 72 h, and oligosaccharide yield rate of >40%. Most oligosaccharides were in the form of Nano-size particles [10-500 nm]. Kolmogorov-Smirnov Goodness of Fit Test (KS test) showed that the distribution of the oligosaccharide micro-particles fitted a lognormal model (p > .05). Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) analysis showed that the oligosaccharides were mainly comprised of glucose polymer with degrees of polymerization (DP) of 4-17. A. niger 1805 is a potential tool to produce oligosaccharides from WCP.