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3D bioprinted alginate-gelatin based scaffolds for soft tissue engineering
Dipul Chawla1, Tejinder Kaur1, Akshay Joshi1
1Centre for Biomedical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India.
International Journal of Biological Macromolecules
|December 21, 2019
Summary
This study developed a 3D Alginate-Gelatin scaffold for osteochondral tissue regeneration. Optimized scaffolds with 20-25% infill enhanced osteoblast viability and proliferation, balancing biomechanics and regeneration.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Osteoarthritis prevalence necessitates advanced tissue regeneration strategies.
- Developing 3D scaffolds for osteochondral applications faces challenges in biomaterial selection and mechanical property mimicry.
- Mimicking natural tissue formation via cell encapsulation in 3D constructs can enhance mechanical properties through matrix secretion.
Purpose of the Study:
- To optimize an Alginate-Gelatin 3D scaffold for osteochondral tissue regeneration.
- To investigate the effect of different ink concentrations and infill percentages on scaffold properties and cell behavior.
- To evaluate the viability and proliferation of osteoblast cells within the developed scaffolds.
Main Methods:
- Fabrication and characterization of Alginate-Gelatin 3D scaffolds at varying concentrations and infill percentages.
- Encapsulation of osteoblast (MG63) cells within the scaffolds.
- Assessment of scaffold printability, viscosity, swelling, bioactivity, and morphology.
- Evaluation of cell viability and proliferation using optimized scaffold parameters.
- Application of dual crosslinking for shape retention.
Main Results:
- An optimized Alginate-Gelatin concentration (2.5% w/v Alginate and 5% w/v Gelatin) demonstrated good printability, viscosity, and swelling.
- Scaffolds with 20% and 25% infill exhibited enhanced osteoblast viability and proliferation.
- Dual crosslinking improved the shape retention ability of the scaffolds.
- The developed 3D scaffolds successfully balanced biomechanical properties with soft tissue regeneration potential.
Conclusions:
- The optimized Alginate-Gelatin 3D scaffold shows promise for osteochondral tissue regeneration.
- Scaffold design parameters, including infill percentage, significantly influence cell behavior and regenerative capacity.
- The developed biomaterial construct offers a viable approach for addressing the need for effective osteoarthritis treatments.
